Recombinant Anti-SOX1 antibody [EPR4766] (ab109290)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4766] to SOX1
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SOX1 antibody [EPR4766]
See all SOX1 primary antibodies -
Description
Rabbit monoclonal [EPR4766] to SOX1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4766 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
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ICC/IF | (1) |
1/100.
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Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Transcriptional activator. -
Tissue specificity
Mainly expressed in the developing central nervous system. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6656 Human
- Entrez Gene: 20664 Mouse
- Omim: 602148 Human
- SwissProt: O00570 Human
- SwissProt: P53783 Mouse
- Unigene: 202526 Human
- Unigene: 39088 Mouse
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Alternative names
- Sex determining region Y box 1 antibody
- sox1 antibody
- SOX1_HUMAN antibody
see all
Images
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All lanes : Anti-SOX1 antibody [EPR4766] (ab109290) at 1/1000 dilution (Purified)
Lane 1 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Lane 4 : Rat cerebral cortex lysate
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 39 kDa -
Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labelling SOX1 with Purified ab109290 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling SOX1 with Purified ab109290 at 1:500 dilution (2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab109290 has been referenced in 16 publications.
- Luo C et al. CircFBXW7 inhibits the tumorigenesis of T-cell acute lymphoblastic leukemia through modulating miR-494-3p/SOX1 axis. Cell Death Discov 8:256 (2022). PubMed: 35538053
- Shen X et al. Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway. BMC Biol 20:219 (2022). PubMed: 36199093
- Xu L et al. Neural stemness contributes to cell tumorigenicity. Cell Biosci 11:21 (2021). PubMed: 33468253
- Karahuseyinoglu S et al. Three-dimensional neuron-astrocyte construction on matrigel enhances establishment of functional voltage-gated sodium channels. J Neurochem 156:848-866 (2021). PubMed: 32939791
- Zhang J et al. Effect of NTN and Lmx1α on the Notch Signaling Pathway during the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Dopaminergic Neuron-Like Cells. Parkinsons Dis 2021:6676709 (2021). PubMed: 34373779