Recombinant human ERK1 protein (ab116536)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% Proprietary Purification
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, MS
Description
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Product name
Recombinant human ERK1 protein
See all ERK1 proteins and peptides -
Biological activity
Specific activity: 379.25 units/ml; 621.73 Units/mg
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Purity
> 80 % Proprietary Purification. -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAAAAAQGGGGGEPRRTEGV GPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAI KKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYI VQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPS NLLSNTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSK GYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLN CIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRIT VEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETAR FQPGVLEAP -
Predicted molecular weight
70 kDa including tags -
Amino acids
2 to 379
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab116536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
Mass Spectrometry
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Mass spectrometry
MALDI-TOF-TOF -
Form
Liquid -
Additional notes
ab116536 was alkylated, digested with trypsin and the mass of the resultant peptides determined by MADLI-TOF/TOF. The peptides obtained gave 27% sequence coverage of ERK1. Theoretical pI: 6.06 -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.002% Brij, 0.012% Benzamidine, 0.003% EGTA, 0.003% PMSF, 0.1% Beta mercaptoethanol, 0.79% Tris HCl, 9.24% Sucrose, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- ERK 1
- ERK-1
- ERK1
see all -
Function
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab116536 has been referenced in 2 publications.
- Sinkala E & Herr AE Single-Cell Western Blotting. Methods Mol Biol 1346:1-9 (2015). PubMed: 26542711
- Hughes AJ et al. Single-cell western blotting. Nat Methods 11:749-55 (2014). PubMed: 24880876