Anti-B4GALT1 antibody (ab121326)
Key features and details
- Rabbit polyclonal to B4GALT1
- Suitable for: ICC, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-B4GALT1 antibody -
Description
Rabbit polyclonal to B4GALT1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 50-163 of Human B4GALT1 (P15291).
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Positive control
- IHC-P: Human placenta, prostate, and testis tissue sections; WB: HeLa cell lysate; ICC: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix. -
Tissue specificity
Ubiquitously expressed, but at very low levels in fetal and adult brain. -
Pathway
Protein modification; protein glycosylation. -
Involvement in disease
Defects in B4GALT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D) [MIM:607091]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. -
Sequence similarities
Belongs to the glycosyltransferase 7 family. -
Post-translational
modificationsThe soluble form derives from the membrane forms by proteolytic processing. -
Cellular localization
Golgi apparatus > Golgi stack membrane. Cell membrane. Cell surface. Found in trans cisternae of Golgi; Golgi apparatus > Golgi stack membrane. Found in trans cisternae of Golgi and Secreted. Soluble form found in body fluids. - Information by UniProt
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Database links
- Entrez Gene: 2683 Human
- Omim: 137060 Human
- SwissProt: P15291 Human
- Unigene: 272011 Human
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Alternative names
- 384.Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase antibody
- 4-galactosyltransferase 1 antibody
- 4-GalTase 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry analysis of A431 (human epidermoid carcinoma) cells labelling B4GALT1 with ab121326 at 2 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-B4GALT1 antibody (ab121326) at 2 µg
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysateLoading control: Anti-HSP90B1
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling B4GALT1 with ab121326 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab121326 has been referenced in 6 publications.
- De Vitis C et al. B4GALT1 as a New Biomarker of Idiopathic Pulmonary Fibrosis. Int J Mol Sci 23:N/A (2022). PubMed: 36499368
- Ren Z et al. High expression of B4GALT1 is associated with poor prognosis in acute myeloid leukemia. Front Genet 13:882004 (2022). PubMed: 36568388
- Chen Y et al. Galactosyltransferase B4GALT1 confers chemoresistance in pancreatic ductal adenocarcinomas by upregulating N-linked glycosylation of CDK11p110. Cancer Lett 500:228-243 (2021). PubMed: 33309857
- Srivastava T et al. Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes. Am J Physiol Renal Physiol 319:F312-F322 (2020). PubMed: 32628542
- Ma X et al. Identification of a distinct luminal subgroup diagnosing and stratifying early stage prostate cancer by tissue-based single-cell RNA sequencing. Mol Cancer 19:147 (2020). PubMed: 33032611
- Wu J et al. ZFX modulates the growth of human leukemic cells via B4GALT1. Acta Biochim Biophys Sin (Shanghai) 48:1120-1127 (2016). PubMed: 27797721