Recombinant Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6766(B)] to Desmoglein 1/DSG1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)]
See all Desmoglein 1/DSG1 primary antibodies -
Description
Rabbit monoclonal [EPR6766(B)] to Desmoglein 1/DSG1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Desmoglein 1/DSG1 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q02413 -
Positive control
- WB: Human skin lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6766(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 114 kDa.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 114 kDa. |
Target
-
Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. -
Tissue specificity
Epidermis, tongue, tonsil and esophagus. -
Involvement in disease
Defects in DSG1 are the cause of palmoplantar keratoderma striate type 1 (SPPK1) [MIM:148700]; also known as keratosis palmoplantaris striata I. SPPK1 is a dermatoligical disorder characterized by thickening of the skin on the palms and soles, and longitudinal hyperkeratotic lesions on the palms, running the length of each finger. -
Sequence similarities
Contains 4 cadherin domains. -
Domain
Calcium may be bound by the cadherin-like repeats. -
Cellular localization
Cell membrane. Cell junction > desmosome. - Information by UniProt
-
Database links
- Entrez Gene: 1828 Human
- Omim: 125670 Human
- SwissProt: Q02413 Human
- Unigene: 2633 Human
-
Alternative names
- Cadherin family member 4 antibody
- CDHF4 antibody
- Desmoglein-1 antibody
see all
Images
-
All lanes : Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/10000 dilution
Lane 1 : Human skin lysate prepared in RIPA lysis method
Lane 2 : Human skin lysate prepared in 1%SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution
Predicted band size: 114 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
We recommend using 1%SDS Hot lysis prepare method to get desired Western Blot results.
-
Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution + Human skin lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 114 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (13)
ab124798 has been referenced in 13 publications.
- Cohen-Barak E et al. Acral peeling in Nagashima type palmo-plantar keratosis patients reveals the role of serine protease inhibitor B 7 in keratinocyte adhesion. Exp Dermatol 31:214-222 (2022). PubMed: 34379845
- Uberoi A et al. Commensal microbiota regulates skin barrier function and repair via signaling through the aryl hydrocarbon receptor. Cell Host Microbe 29:1235-1248.e8 (2021). PubMed: 34214492
- Yi L et al. Emodin inhibits viability, proliferation and promotes apoptosis of hypoxic human pulmonary artery smooth muscle cells via targeting miR-244-5p/DEGS1 axis. BMC Pulm Med 21:252 (2021). PubMed: 34332565
- Yoon JS et al. Biophysical characteristics of dandruff-affected scalp categorized on the basis of sebum levels. J Cosmet Dermatol 20:1002-1008 (2021). PubMed: 32757243
- Kashgari G et al. Epithelial Migration and Non-adhesive Periderm Are Required for Digit Separation during Mammalian Development. Dev Cell 52:764-778.e4 (2020). PubMed: 32109382