Recombinant Anti-FAAH1 antibody [EPR7549] (ab128917)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7549] to FAAH1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-FAAH1 antibody [EPR7549]
See all FAAH1 primary antibodies -
Description
Rabbit monoclonal [EPR7549] to FAAH1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human FAAH1 aa 500-600. The exact sequence is proprietary.
-
Positive control
- Human testis, JAR and A431 cell lysates; Human brain and kidney tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7549 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
|
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is highly recommended. |
Notes |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is highly recommended. |
Target
-
Function
Degrades bioactive fatty acid amides like oleamide, the endogenous cannabinoid, anandamide and myristic amide to their corresponding acids, thereby serving to terminate the signaling functions of these molecules. Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates. -
Tissue specificity
Highly expressed in the brain, small intestine, pancreas, skeletal muscle and testis. Also expressed in the kidney, liver, lung, placenta and prostate. -
Sequence similarities
Belongs to the amidase family. -
Cellular localization
Endomembrane system. Cytoplasm > cytoskeleton. Seems to be attached to intracellular membranes and a portion of the cytoskeletal network. - Information by UniProt
-
Database links
- Entrez Gene: 2166 Human
- Entrez Gene: 14073 Mouse
- Omim: 602935 Human
- SwissProt: O00519 Human
- SwissProt: O08914 Mouse
- Unigene: 720143 Human
- Unigene: 256025 Mouse
-
Alternative names
- Anandamide amidohydrolase 1 antibody
- Anandamide amidohydrolase antibody
- FA2H antibody
see all
Images
-
All lanes : Anti-FAAH1 antibody [EPR7549] (ab128917) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : JAR cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 63 kDa -
ab128917, at 1/250 dilution, staining FAAH1 in paraffin embedded Human brain tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab128917, at 1/250 dilution, staining FAAH1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab128917 has been referenced in 5 publications.
- Duncan RS et al. N-acylethanolamide metabolizing enzymes are upregulated in human neural progenitor-derived neurons exposed to sub-lethal oxidative stress. Front Cell Neurosci 16:902278 (2022). PubMed: 36003139
- Li H et al. MicroRNA-1297 downregulation inhibits breast cancer cell epithelial-mesenchymal transition and proliferation in a FA2H-dependent manner. Oncol Lett 20:277 (2020). PubMed: 33014155
- Hong B et al. miR-300/FA2H affects gastric cancer cell proliferation and apoptosis. Open Med (Wars) 15:882-889 (2020). PubMed: 33344772
- Zhang K et al. CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice. J Clin Invest 129:2333-2350 (2019). PubMed: 31063987
- Aichler M et al. N-acyl Taurines and Acylcarnitines Cause an Imbalance in Insulin Synthesis and Secretion Provoking ß Cell Dysfunction in Type 2 Diabetes. Cell Metab 25:1334-1347.e4 (2017). PubMed: 28591636