Recombinant Anti-ERCC1 antibody [EPR7062] (ab129093)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7062] to ERCC1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ERCC1 antibody [EPR7062]
See all ERCC1 primary antibodies -
Description
Rabbit monoclonal [EPR7062] to ERCC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human ERCC1 aa 150-300. The exact sequence is proprietary.
Database link: P07992 -
Positive control
- HeLa, SKBR-3, and 293T cell lysates, Human lung carcinoma tissue, HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7062 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
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IHC-P |
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. -
Involvement in disease
Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) [MIM:610758]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. -
Sequence similarities
Belongs to the ERCC1/RAD10/SWI10 family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2067 Human
- Entrez Gene: 13870 Mouse
- Entrez Gene: 292673 Rat
- Omim: 126380 Human
- SwissProt: P07992 Human
- SwissProt: P07903 Mouse
- Unigene: 435981 Human
- Unigene: 280913 Mouse
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Alternative names
- COFS 4 antibody
- COFS4 antibody
- DNA excision repair protein ERCC 1 antibody
see all
Images
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All lanes : Anti-ERCC1 antibody [EPR7062] (ab129093) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : SKBR-3 cell lysates
Lane 3 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for ERCC1 with ab129093 (1/25 dilution) in immunohistochemical analysis.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab129093, at a 1/100 dilution, staining ERCC1 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab129093 has not yet been referenced specifically in any publications.