Recombinant Anti-NAPSIN A antibody [EPR6257] (ab129189)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6257] to NAPSIN A
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NAPSIN A antibody [EPR6257]
See all NAPSIN A primary antibodies -
Description
Rabbit monoclonal [EPR6257] to NAPSIN A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NAPSIN A aa 50-150. The exact sequence is proprietary.
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Positive control
- WB: Human lung adenocarcinoma lysate. IHC-P: Human lung tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.17 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6257 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
May be involved in processing of pneumocyte surfactant precursors. -
Tissue specificity
Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9476 Human
- Omim: 605631 Human
- SwissProt: O96009 Human
- Unigene: 512843 Human
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Alternative names
- Asp 4 antibody
- ASP4 antibody
- Aspartyl protease 4 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling NAPSIN A with purified ab129189 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Anti-NAPSIN A antibody [EPR6257] (ab129189) at 1/5000 dilution (purified) + Human lung adenocarcinoma tissue lysate at 20 µg
Secondary
HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM /TBST.
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Anti-NAPSIN A antibody [EPR6257] (ab129189) at 1/1000 dilution (unpurified) + Human lung adenocarcinoma lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 45 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling NAPSIN A with unpurified ab129189 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab129189 has not yet been referenced specifically in any publications.