Anti-Serotonin transporter antibody (ab130130)
Key features and details
- Goat polyclonal to Serotonin transporter
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Serotonin transporter antibody
See all Serotonin transporter primary antibodies -
Description
Goat polyclonal to Serotonin transporter -
Host species
Goat -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide corresponding to Human Serotonin transporter aa 605-618 (C terminal) (Cysteine residue). (NP_001036.1)
Sequence:C-KERIIKSITPETPT
Database link: P31645 -
Positive control
- ICC: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab130130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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Notes |
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ICC
Use at an assay dependent concentration. |
Target
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Function
Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner. -
Tissue specificity
Expressed in platelets (at protein level). -
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily. -
Post-translational
modificationsGlycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms.
Phosphorylated at Ser-611, Thr-613 and Thr-616. -
Cellular localization
Cell membrane. Endomembrane system. Endosome membrane. Translocates from intracellular locations to the plasma membrane. Density of transporter molecules on the plasma membrane is itself regulated by serotonin. - Information by UniProt
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Database links
- Entrez Gene: 282365 Cow
- Entrez Gene: 6532 Human
- Entrez Gene: 15567 Mouse
- Entrez Gene: 25553 Rat
- Omim: 182138 Human
- SwissProt: Q9XT49 Cow
- SwissProt: P31645 Human
- SwissProt: Q60857 Mouse
see all -
Alternative names
- 5 HTT antibody
- 5 HTTLPR antibody
- 5 hydroxytryptamine (serotonin) transporter antibody
see all
Images
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Immunocytochemistry analysis of paraformaldehyde fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Serotonin transporter with ab130130 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL.
Datasheets and documents
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Datasheet download
References (11)
ab130130 has been referenced in 11 publications.
- Layunta E et al. Intestinal serotonergic system is modulated by Toll-like receptor 9. J Physiol Biochem 78:689-701 (2022). PubMed: 35670957
- Kingston R et al. Serotonin transporter-mediated molecular axis regulates regional retinal ganglion cell vulnerability and axon regeneration after nerve injury. PLoS Genet 17:e1009885 (2021). PubMed: 34735454
- Lee JS et al. An Adrenalectomy Mouse Model Reflecting Clinical Features for Chronic Fatigue Syndrome. Biomolecules 10:N/A (2020). PubMed: 31906307
- Cheng Q et al. [6]-Gingerol Ameliorates Cisplatin-Induced Pica by Regulating the TPH/MAO-A/SERT/5-HT/5-HT3 Receptor System in Rats. Drug Des Devel Ther 14:4085-4099 (2020). PubMed: 33061309
- Li C et al. Modulation of depression-related behaviors by adiponectin AdipoR1 receptors in 5-HT neurons. Mol Psychiatry N/A:N/A (2020). PubMed: 31980728