Anti-proBNP antibody [5B6] (ab13111)
Key features and details
- Mouse monoclonal [5B6] to proBNP
- Suitable for: ELISA, WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-proBNP antibody [5B6]
See all proBNP primary antibodies -
Description
Mouse monoclonal [5B6] to proBNP -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Synthetic peptide:
HPLGSPGSASDL
, corresponding to amino acids 1 - 12 of Human proBNP. -
General notes
This product has switched from ascites to TCS on 10th June 2020.
Immunoglobulin concentrations vary from batch to batch (generally 4-6 mg/ml for this clone). Please contact technical services for the latest information.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5B6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 13 kDa.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 13 kDa. |
Target
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Function
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Tissue specificity
Brain and also in atria, but at much lower levels than ANP. -
Sequence similarities
Belongs to the natriuretic peptide family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANFB_HUMAN antibody
- BNP antibody
- BNP(1-32) antibody
see all
Images
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WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab13111 has been referenced in 4 publications.
- Hsu A et al. Targeting transcription in heart failure via CDK7/12/13 inhibition. Nat Commun 13:4345 (2022). PubMed: 35896549
- Hsu A et al. Salt-inducible kinase 1 maintains HDAC7 stability to promote pathologic cardiac remodeling. J Clin Invest 130:2966-2977 (2020). PubMed: 32106109
- Duan Q et al. BET bromodomain inhibition suppresses innate inflammatory and profibrotic transcriptional networks in heart failure. Sci Transl Med 9:N/A (2017). PubMed: 28515341
- Carlson C et al. Phenotypic Screening with Human iPS Cell-Derived Cardiomyocytes: HTS-Compatible Assays for Interrogating Cardiac Hypertrophy. J Biomol Screen N/A:N/A (2013). PubMed: 24071917