Recombinant Anti-ATP7b antibody [EPR6793] (ab131208)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6793] to ATP7b
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATP7b antibody [EPR6793]
See all ATP7b primary antibodies -
Description
Rabbit monoclonal [EPR6793] to ATP7b -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Caco-2 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 6.79 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6793 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 157 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 157 kDa. |
Target
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Function
Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. -
Tissue specificity
Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. -
Involvement in disease
Defects in ATP7B are the cause of Wilson disease (WD) [MIM:277900]. WD is an autosomal recessive disorder of copper metabolism in which copper cannot be incorporated into ceruloplasmin in liver, and cannot be excreted from the liver into the bile. Copper accumulates in the liver and subsequently in the brain and kidney. The disease is characterized by neurologic manifestations and signs of cirrhosis. -
Sequence similarities
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.
Contains 6 HMA domains. -
Post-translational
modificationsIsoform 1 may be proteolytically cleaved at the N-terminus to produce the WND/140 kDa form. -
Cellular localization
Cytoplasm; Mitochondrion and Golgi apparatus > trans-Golgi network membrane. Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels. - Information by UniProt
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Database links
- Entrez Gene: 540 Human
- Omim: 606882 Human
- SwissProt: P35670 Human
- Unigene: 492280 Human
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Alternative names
- ATP7B antibody
- ATP7B_HUMAN antibody
- ATPase, Cu(2+) transporting, beta polypeptide antibody
see all
Images
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Anti-ATP7b antibody [EPR6793] (ab131208) at 1/1000 dilution + Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 157 kDa
Observed band size: 165 kDa why is the actual band size different from the predicted?The molecular weight observed is consistent with what has been described in PMID: 26438057 and 18673401
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab131208 has been referenced in 2 publications.
- Gao W et al. Elesclomol induces copper-dependent ferroptosis in colorectal cancer cells via degradation of ATP7A. Mol Oncol 15:3527-3544 (2021). PubMed: 34390123
- Merico D et al. ATP7B variant c.1934T?>?G p.Met645Arg causes Wilson disease by promoting exon 6 skipping. NPJ Genom Med 5:16 (2020). PubMed: 32284880