Recombinant Anti-HOXB4 antibody [EPR1917] (ab133521)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1917] to HOXB4
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-HOXB4 antibody [EPR1917]
See all HOXB4 primary antibodies -
Description
Rabbit monoclonal [EPR1917] to HOXB4 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human HOXB4 aa 1-100. The exact sequence is proprietary.
-
Positive control
- K562, Jurkat, RAW 264.7, and PC-12 cell lysates; NCCIT cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.49 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1917 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100 - 1/200.
|
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 34 kDa.
|
Notes |
---|
ICC/IF
1/100 - 1/200. |
WB
1/1000 - 1/10000. Predicted molecular weight: 34 kDa. |
Target
-
Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
Sequence similarities
Belongs to the Antp homeobox family. Deformed subfamily.
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed in whole embryos and fetuses at 5-9 weeks from conception. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 3214 Human
- Entrez Gene: 15412 Mouse
- Entrez Gene: 497988 Rat
- Omim: 142965 Human
- SwissProt: P17483 Human
- SwissProt: P10284 Mouse
- Unigene: 664706 Human
- Unigene: 3546 Mouse
-
Alternative names
- Homeo box 2F antibody
- Homeo box B4 antibody
- Homeobox 2F antibody
see all
Images
-
All lanes : Anti-HOXB4 antibody [EPR1917] (ab133521) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : RAW 264.7 cell lysate
Lane 4 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 34 kDa -
Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma cell line) cells labeling HOXB4 with ab133521 at 1/100 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab133521 has been referenced in 4 publications.
- Tay SH et al. Generation of Cortical, Dopaminergic, Motor, and Sensory Neurons from Human Pluripotent Stem Cells. Methods Mol Biol 2549:359-377 (2022). PubMed: 33959917
- Walsh P et al. Accelerated differentiation of human pluripotent stem cells into neural lineages via an early intermediate ectoderm population. Stem Cells 38:1400-1408 (2020). PubMed: 32745311
- Lim GS et al. Microhexagon gradient array directs spatial diversification of spinal motor neurons. Theranostics 9:311-323 (2019). PubMed: 30809276
- Hor JH et al. Cell cycle inhibitors protect motor neurons in an organoid model of Spinal Muscular Atrophy. Cell Death Dis 9:1100 (2018). PubMed: 30368521