Recombinant Anti-Hemopexin antibody [EPR5609] (ab133523)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5609] to Hemopexin
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Hemopexin antibody [EPR5609]
See all Hemopexin primary antibodies -
Description
Rabbit monoclonal [EPR5609] to Hemopexin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human plasma and placenta, fetal liver, and Human thyroid lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.40 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5609 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/50000 - 1/200000. Detects a band of approximately 70-75 kDa (predicted molecular weight: 51 kDa).
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Notes |
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WB
1/50000 - 1/200000. Detects a band of approximately 70-75 kDa (predicted molecular weight: 51 kDa). |
Target
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Function
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
Post-translational
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3263 Human
- Omim: 142290 Human
- SwissProt: P02790 Human
- Unigene: 426485 Human
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Alternative names
- Beta 1B glycoprotein antibody
- Beta-1B-glycoprotein antibody
- FLJ56652 antibody
see all
Images
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All lanes : Anti-Hemopexin antibody [EPR5609] (ab133523) at 1/50000 dilution
Lane 1 : Human plasma lysate
Lane 2 : Human placenta lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human thyroid lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 70-75 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133523 has not yet been referenced specifically in any publications.