Recombinant Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292]
See all Glucose 6 Phosphate Dehydrogenase primary antibodies -
Description
Rabbit monoclonal [EPR6292] to Glucose 6 Phosphate Dehydrogenase -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Molt 4, A549, MCF7, HeLa, and HepG2 cell lysates. IHC-P: Human testis tissue. Flow Cyt (intra): A549 cells
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General notes
Cellular localisation: centrosome, cytosol, internal side of plasma membrane, intracellular membrane bounded organelle. There are 3 isoforms produced by alternative splicing.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6292 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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WB |
1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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WB
1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. -
Tissue specificity
Isoform Long is found in lymphoblasts, granulocytes and sperm. -
Pathway
Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. -
Involvement in disease
Anemia, non-spherocytic hemolytic, due to G6PD deficiency -
Sequence similarities
Belongs to the glucose-6-phosphate dehydrogenase family. -
Post-translational
modificationsAcetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. - Information by UniProt
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Database links
- Entrez Gene: 2539 Human
- Omim: 305900 Human
- SwissProt: P11413 Human
- Unigene: 461047 Human
- Unigene: 684904 Human
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Alternative names
- G6PD antibody
- G6PD_HUMAN antibody
- G6PD1 antibody
see all
Images
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All lanes : Anti-Glucose 6 Phosphate Dehydrogenase antibody [EPR6292] (ab133525) at 1/1000 dilution
Lane 1 : Molt 4 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit at 1/2000 dilution
Predicted band size: 59 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted? -
Immunohistochemistry analysis of Paraffin Embedded Human testis tissue labelling Glucose 6 Phosphate Dehydrogenase with ab133525 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling Glucose 6 Phosphate Dehydrogenase with purified ab133525 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab133525 has been referenced in 15 publications.
- Zhang Q et al. G6PD upregulates Cyclin E1 and MMP9 to promote clear cell renal cell carcinoma progression. Int J Med Sci 19:47-64 (2022). PubMed: 34975298
- Mohás A et al. In Situ Analysis of mTORC1/C2 and Metabolism-Related Proteins in Pediatric Osteosarcoma. Pathol Oncol Res 28:1610231 (2022). PubMed: 35392503
- Lv Y et al. BRD4 Targets the KEAP1-Nrf2-G6PD Axis and Suppresses Redox Metabolism in Small Cell Lung Cancer. Antioxidants (Basel) 11:N/A (2022). PubMed: 35453346
- Wang H et al. Nuclear TIGAR mediates an epigenetic and metabolic autoregulatory loop via NRF2 in cancer therapeutic resistance. Acta Pharm Sin B 12:1871-1884 (2022). PubMed: 35847493
- Ni Y et al. Silent information regulator 2 promotes clear cell renal cell carcinoma progression through deacetylation and small ubiquitin-related modifier 1 modification of glucose 6-phosphate dehydrogenase. Cancer Sci 112:4075-4086 (2021). PubMed: 34310804