Recombinant Anti-PEF1 antibody [EPR9310] (ab137127)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9310] to PEF1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PEF1 antibody [EPR9310]
See all PEF1 primary antibodies -
Description
Rabbit monoclonal [EPR9310] to PEF1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human PEF1 aa 100-200. The exact sequence is proprietary.
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Positive control
- Jurkat, HeLa, 293T, and HL60 cell lysates; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9310 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137127 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa. |
Target
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Sequence similarities
Contains 5 EF-hand domains. -
Cellular localization
Cytoplasm. Membrane. Membrane-associated in the presence of C. - Information by UniProt
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Database links
- Entrez Gene: 553115 Human
- Entrez Gene: 67898 Mouse
- Entrez Gene: 297900 Rat
- Omim: 610033 Human
- SwissProt: Q9UBV8 Human
- SwissProt: Q8BFY6 Mouse
- SwissProt: Q641Z8 Rat
- Unigene: 470417 Human
see all -
Alternative names
- ABP32 antibody
- PEF antibody
- PEF protein with a long N terminal hydrophobic domain antibody
see all
Images
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All lanes : Anti-PEF1 antibody [EPR9310] (ab137127) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HL60 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 30 kDa -
Intracellular flow cytometric analysis of permeabilized 293T cells labelling PEF1 with ab137127 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab137127 has been referenced in 4 publications.
- Loers G et al. Interplay in neural functions of cell adhesion molecule close homolog of L1 (CHL1) and Programmed Cell Death 6 (PDCD6). FASEB Bioadv 4:43-59 (2022). PubMed: 35024572
- Akopian D et al. Co-adaptor driven assembly of a CUL3 E3 ligase complex. Mol Cell 82:585-597.e11 (2022). PubMed: 35120648
- Mena EL et al. Structural basis for dimerization quality control. Nature 586:452-456 (2020). PubMed: 32814905
- McGourty CA et al. Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor. Cell 167:525-538.e14 (2016). PubMed: 27716508