DCFA (5(6)-Carboxy-2',7'-dichlorofluorescein), green fluorophore (ab145439)
Key features and details
- Green fluorophore. Intracellular pH indicator in the pH 4-5 range.
- CAS Number: 111843-78-8
- Purity: > 95%
- Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
Overview
-
Product name
DCFA (5(6)-Carboxy-2',7'-dichlorofluorescein), green fluorophore -
Description
Green fluorophore. Intracellular pH indicator in the pH 4-5 range. -
Biological description
-
Purity
> 95% -
CAS Number
111843-78-8 -
Chemical structure
Properties
-
Excitation
495nm -
Emission
529nm -
Chemical name
5(6)-Carboxy-2',7'-dichlorofluorescein -
Molecular weight
445.21 -
Molecular formula
C21H10Cl2O7 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
Source
Synthetic
-
Research areas
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab145439 has been referenced in 1 publication.
- Boul M et al. A versatile microfluidic tool for the 3D culture of HepaRG cells seeded at various stages of differentiation. Sci Rep 11:14075 (2021). PubMed: 34234159