Anti-Nucleolin antibody [3G4B2] (ab14966)
Key features and details
- Mouse monoclonal [3G4B2] to Nucleolin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
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- Success from the first experiment – confirmed specificity through extensive validation
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Overview
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Product name
Anti-Nucleolin antibody [3G4B2]
See all Nucleolin primary antibodies -
Description
Mouse monoclonal [3G4B2] to Nucleolin -
Host species
Mouse -
Specificity
Recognizes human nucleolin, MW 100kD and an additional protein, MW 95kDa in HeLa nuclear extract. The clone number has been updated from (4i51) to (3G4B2) both clone numbers name the same antibody clone. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Nuclear matrix extract from MDCK cells (dog).
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Positive control
- WB: HeLa nuclear extract. IP: MDCK total cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 30% Glycerol
Supplied as a liquid in 100µl of culture supernatant containing 0.05% sodium azide prior to the addition of glycerol to 30%. -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
3G4B2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 85 kDa).
A weak non-specific band of ~60kDa is detected in human cell lines with lower antibody dilutions. |
Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 106 kDa (predicted molecular weight: 85 kDa). A weak non-specific band of ~60kDa is detected in human cell lines with lower antibody dilutions. |
Target
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Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Post-translational
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Database links
- Entrez Gene: 4691 Human
- Omim: 164035 Human
- SwissProt: P19338 Human
- Unigene: 79110 Human
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Alternative names
- C23 antibody
- FLJ45706 antibody
- MS1116 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab14966 has been referenced in 5 publications.
- Baez M et al. Analysis of the small chromosomal Prionium serratum (Cyperid) demonstrates the importance of reliable methods to differentiate between mono- and holocentricity. Chromosoma 129:285-297 (2020). PubMed: 33165742
- Wang Y et al. PNRC accumulates in the nucleolus by interaction with B23/nucleophosmin via its nucleolar localization sequence. Biochim Biophys Acta : (2010). PubMed: 20888865
- Hwang J et al. Role of Scarf and its binding target proteins in epidermal calcium homeostasis. J Biol Chem 282:18645-53 (2007). IHC-P, IP . PubMed: 17470426
- Huddleson JP et al. Up-regulation of the KLF2 transcription factor by fluid shear stress requires nucleolin. J Biol Chem 281:15121-8 (2006). WB, EMSA ; Human . PubMed: 16571724
- Gotzmann J et al. Two-dimensional electrophoresis reveals a nuclear matrix-associated nucleolin complex of basic isoelectric point. Electrophoresis 18:2645-53 (1997). IP ; Human . PubMed: 9527495