Anti-Carbonic Anhydrase 3/CA3 antibody (ab150037)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 3/CA3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Carbonic Anhydrase 3/CA3 antibody
See all Carbonic Anhydrase 3/CA3 primary antibodies -
Description
Rabbit polyclonal to Carbonic Anhydrase 3/CA3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee, Macaque monkey, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Carbonic Anhydrase 3/CA3 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab136233) -
Positive control
- This antibody gave a positive signal in Recombinant Carbonic anhydrase 3 protein as well as the following tissue lysates: Human Skeletal Muscle; Mouse Skeletal Muscle; Rat Skeletal Muscle. It also gave a positive result in FFPE human skeletal muscle tissue sections.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
|
|
IHC-P |
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa). |
IHC-P
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Reversible hydration of carbon dioxide. -
Tissue specificity
Muscle specific. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family. -
Developmental stage
At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord. -
Post-translational
modificationsS-glutathionylated in hepatocytes under oxidative stress. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 761 Human
- Entrez Gene: 12350 Mouse
- Entrez Gene: 54232 Rat
- Omim: 114750 Human
- SwissProt: P07451 Human
- SwissProt: P16015 Mouse
- SwissProt: P14141 Rat
- Unigene: 82129 Human
see all -
Alternative names
- CA III antibody
- CA-III antibody
- Ca3 antibody
see all
Images
-
All lanes : Anti-Carbonic Anhydrase 3/CA3 antibody (ab150037) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein
Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lane 4 : Carbonic Anhydrase III/CA3 protein
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 24 kDa, 58 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds -
IHC image of Carbonic Anydrase III staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab150037, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab150037 has not yet been referenced specifically in any publications.