Anti-Dynamin 1 antibody (ab150055)
Key features and details
- Rabbit polyclonal to Dynamin 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Dynamin 1 antibody
See all Dynamin 1 primary antibodies -
Description
Rabbit polyclonal to Dynamin 1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey -
Immunogen
Synthetic peptide corresponding to Human Dynamin 1 aa 600-700 conjugated to keyhole limpet haemocyanin.
(Peptide available asab139745) -
Positive control
- This antibody gave a positive signal in Human, Mouse and Rat Brain tissue lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 97 kDa).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 97 kDa). |
Target
-
Function
Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis. -
Sequence similarities
Belongs to the dynamin family.
Contains 1 GED domain.
Contains 1 PH domain. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Microtubule-associated. - Information by UniProt
-
Database links
- Entrez Gene: 1759 Human
- Entrez Gene: 13429 Mouse
- Entrez Gene: 140694 Rat
- Omim: 602377 Human
- SwissProt: Q05193 Human
- SwissProt: P39053 Mouse
- SwissProt: P21575 Rat
- Unigene: 522413 Human
see all -
Alternative names
- B dynamin antibody
- D100 antibody
- DNM 1 antibody
see all
Images
-
All lanes : Anti-Dynamin 1 antibody (ab150055) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 102 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa, 95 kDa, 99 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab150055 has been referenced in 1 publication.
- Li J et al. Xanthan Gum Ameliorates Osteoarthritis and Mitigates Cartilage Degradation via Regulation of the Wnt3a/ß-Catenin Signaling Pathway. Med Sci Monit 25:7488-7498 (2019). PubMed: 31587011