Recombinant Anti-PPWD1 antibody [EPR7439] (ab150356)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7439] to PPWD1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-PPWD1 antibody [EPR7439]
See all PPWD1 primary antibodies -
Description
Rabbit monoclonal [EPR7439] to PPWD1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human PPWD1 aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa, NIH3T3, and 293T cell lysates, HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7439 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150356 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa). |
Target
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Function
Putative peptidylprolyl isomerase (PPIase). PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. May be involved in pre-mRNA splicing. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIL1 subfamily.
Contains 1 PPIase cyclophilin-type domain.
Contains 4 WD repeats. -
Cellular localization
Nucleus. Associated with spliceosomal complexes. - Information by UniProt
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Database links
- Entrez Gene: 23398 Human
- Entrez Gene: 238831 Mouse
- Entrez Gene: 294711 Rat
- SwissProt: Q96BP3 Human
- SwissProt: Q8CEC6 Mouse
- Unigene: 121432 Human
- Unigene: 98910 Mouse
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Alternative names
- 4632422M10Rik antibody
- A330090G21Rik antibody
- KIAA0073 antibody
see all
Images
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All lanes : Anti-PPWD1 antibody [EPR7439] (ab150356) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : NIH3T3 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 74 kDa -
Intracellular flow cytometrical analysis of permeabilized HeLa cells with ab150356 antibody at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab150356 has not yet been referenced specifically in any publications.