Recombinant Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9307] to KMT6 / EZH2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-KMT6 / EZH2 antibody [EPR9307]
See all KMT6 / EZH2 primary antibodies -
Description
Rabbit monoclonal [EPR9307] to KMT6 / EZH2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human KMT6/ EZH2 aa 150-300. The exact sequence is proprietary.
Database link: Q15910 -
Positive control
- Recombinant Human KMT6 / EZH2 protein (ab132934) can be used as a positive control in WB. MCF7, Jurkat and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9307 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 85 kDa. |
Target
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Function
Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. -
Tissue specificity
Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis. -
Sequence similarities
Belongs to the histone-lysine methyltransferase family. EZ subfamily.
Contains 1 SET domain. -
Developmental stage
Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary. -
Post-translational
modificationsPhosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2146 Human
- Omim: 601573 Human
- SwissProt: Q15910 Human
- Unigene: 444082 Human
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Alternative names
- Enhancer of zeste 2 antibody
- enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
- Enhancer of zeste homolog 2 (Drosophila) antibody
see all
Images
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Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: KMT6 / EZH2 knockout HAP1 cell lysate (40 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab150433 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab150433 was shown to recognize KMT6 / EZH2 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when KMT6 / EZH2 knockout samples were examined. Wild-type and KMT6 / EZH2 knockout samples were subjected to SDS-PAGE. Ab150433 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4oC. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) (ab216773)
and IRDye® 680 Goat anti-Mouse IgG (H + L) (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-KMT6 / EZH2 antibody [EPR9307] (ab150433) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 85 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab150433 has been referenced in 3 publications.
- Qiu Z et al. CDYL promotes the chemoresistance of small cell lung cancer by regulating H3K27 trimethylation at the CDKN1C promoter. Theranostics 9:4717-4729 (2019). PubMed: 31367252
- Wang J et al. Evaluation of EZH2 expression, BRAF V600E mutation, and CDKN2A/B deletions in epithelioid glioblastoma and anaplastic pleomorphic xanthoastrocytoma. J Neurooncol 144:137-146 (2019). PubMed: 31214915
- Jin Q et al. MicroRNA-101-3p inhibits proliferation in retinoblastoma cells by targeting EZH2 and HDAC9. Exp Ther Med 16:1663-1670 (2018). PubMed: 30186385