Anti-Frizzled 2/FZD2 antibody (ab150477)
Key features and details
- Rabbit polyclonal to Frizzled 2/FZD2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Frizzled 2/FZD2 antibody
See all Frizzled 2/FZD2 primary antibodies -
Description
Rabbit polyclonal to Frizzled 2/FZD2 -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except FZD7 (60%), DMAP1 (40%). -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Human Frizzled 2/FZD2 (N terminal).
Database link: NP_001457.1 -
Positive control
- Human fetal kidney tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 8 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 8 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. -
Tissue specificity
Widely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver. -
Sequence similarities
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain. -
Domain
Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.
The FZ domain is involved in binding with Wnt ligands. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2535 Human
- Entrez Gene: 57265 Mouse
- Entrez Gene: 64512 Rat
- Omim: 600667 Human
- SwissProt: Q14332 Human
- SwissProt: Q9JIP6 Mouse
- SwissProt: Q08464 Rat
- Unigene: 142912 Human
see all -
Alternative names
- Frizzled homolog 2 antibody
- Frizzled-2 antibody
- Frizzled2 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab150477 has been referenced in 1 publication.
- Tao L et al. Frizzled proteins are colonic epithelial receptors for C. difficile toxin B. Nature 538:350-355 (2016). PubMed: 27680706