Anti-Diphtheria Toxin antibody (ab151222)
Key features and details
- Rabbit polyclonal to Diphtheria Toxin
- Suitable for: WB, IP, ELISA, Dot blot
- Isotype: IgG
Overview
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Product name
Anti-Diphtheria Toxin antibody
See all Diphtheria Toxin primary antibodies -
Description
Rabbit polyclonal to Diphtheria Toxin -
Host species
Rabbit -
Specificity
Reacts with Diphtheria Toxin as well as its CRM197 mutant toxin. -
Tested applications
Suitable for: WB, IP, ELISA, Dot blotmore details -
Species reactivity
Reacts with: Corynebacterium diphtheriae -
Immunogen
Purified non-toxic mutant Diphtheria Toxin CRM 197.
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Positive control
- WB: Crude culture medium of Corynebacterium Diphtheria, The two-fold diluted culture medium, Purified CRM197, Purified Diphtheria toxin.
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General notes
For a recombinant antibody to Diphtheria Toxin, please see - ab209329.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Constituent: 100% Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Purification notes
Whole antiserum sterilized by filtration. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Can detect 1 ng or less of the toxin
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IP |
1/200 - 1/1000.
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ELISA |
1/2000 - 1/7000.
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Dot blot |
1/1000. Can detect 1 ng or less of the toxin
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Notes |
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WB
1/2000. Can detect 1 ng or less of the toxin |
IP
1/200 - 1/1000. |
ELISA
1/2000 - 1/7000. |
Dot blot
1/1000. Can detect 1 ng or less of the toxin |
Target
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Relevance
Corynebacterium diphtheriae is a gram-positive, nonmotile bacteria found in soil and animal feces. C. diphteriae infect the epithelial cells of the upper respiratory tract from where they produce and secrete a potent toxin. This toxin is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body. -
Cellular localization
Secreted -
Alternative names
- Corynebacterium diphtheriae toxoid antibody
- Diphtheria Toxin antibody
- tox antibody
Images
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab151222 has been referenced in 3 publications.
- Chai P et al. The recombinant protein combined vaccine based on the fragment C of tetanus toxin and the cross-reacting material 197. Appl Microbiol Biotechnol 105:1683-1692 (2021). PubMed: 33511443
- Purde V et al. Intein-mediated cytoplasmic reconstitution of a split toxin enables selective cell ablation in mixed populations and tumor xenografts. Proc Natl Acad Sci U S A 117:22090-22100 (2020). PubMed: 32839344
- Mishra RPN et al. Structural and immunological characterization of E. coli derived recombinant CRM197 protein used as carrier in conjugate vaccines. Biosci Rep 38:N/A (2018). PubMed: 29875175