Recombinant Anti-beta Crystallin A3 antibody [EPR9586] (ab151722)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9586] to beta Crystallin A3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-beta Crystallin A3 antibody [EPR9586]
See all beta Crystallin A3 primary antibodies -
Description
Rabbit monoclonal [EPR9586] to beta Crystallin A3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Mouse and Rat eyeball lysates; Y79 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9586 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Predicted molecular weight: 25 kDa.
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ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 25 kDa. |
ICC/IF
1/50 - 1/100. |
Target
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Function
Crystallins are the dominant structural components of the vertebrate eye lens. -
Involvement in disease
Defects in CRYBA1 are the cause of cataract congenital zonular with sutural opacities (CCZS) [MIM:600881]. A form of zonular cataract. Zonular or lamellar cataracts are concentric opacities, broad or narrow, usually consisting of powdery white dots affecting one lamella or zonule of an otherwise clear lens. -
Sequence similarities
Belongs to the beta/gamma-crystallin family.
Contains 4 beta/gamma crystallin 'Greek key' domains. -
Domain
Has a two-domain beta-structure, folded into four very similar Greek key motifs. -
Post-translational
modificationsSpecific cleavages in the N-terminal arm occur during lens maturation and give rise to several truncated forms. Cleavages do not seem to have adverse effects on solubility.
S-methylation and glutathionylation occur in normal young lenses and do not seem to be detrimental.
Isoform A1 initiator methionine is removed. The new N-terminal amino acid is then N-acetylated. - Information by UniProt
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Database links
- Entrez Gene: 1411 Human
- Entrez Gene: 12957 Mouse
- Entrez Gene: 25583 Rat
- Omim: 123610 Human
- SwissProt: P05813 Human
- SwissProt: P02525 Mouse
- SwissProt: P14881 Rat
- Unigene: 46275 Human
see all -
Alternative names
- Beta crystallin A3 isoform A1 Delta4 form antibody
- Beta crystallin A3 antibody
- Beta crystallin A3 isoform A1 Delta7 form antibody
see all
Images
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All lanes : Anti-beta Crystallin A3 antibody [EPR9586] (ab151722) at 1/10000 dilution
Lane 1 : Mouse eyeball lysate
Lane 2 : Rat eyeball lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 25 kDa -
Immunofluorescent analysis of Y79 cells labeling beta Crystallin A3 with ab151722 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab151722 has been referenced in 5 publications.
- Ghosh S et al. ßA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activity. Commun Biol 4:248 (2021). PubMed: 33627831
- Shang P et al. ßA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells. Commun Biol 4:850 (2021). PubMed: 34239035
- Lennikov A et al. Autoimmune-Mediated Retinopathy in CXCR5-Deficient Mice as the Result of Age-Related Macular Degeneration Associated Proteins Accumulation. Front Immunol 10:1903 (2019). PubMed: 31474986
- Ghosh S et al. A Role for ßA3/A1-Crystallin in Type 2 EMT of RPE Cells Occurring in Dry Age-Related Macular Degeneration. Invest Ophthalmol Vis Sci 59:AMD104-AMD113 (2018). PubMed: 30098172
- Choudhary P et al. Directing Differentiation of Pluripotent Stem Cells Toward Retinal Pigment Epithelium Lineage. Stem Cells Transl Med 6:490-501 (2017). PubMed: 28191760