Recombinant Anti-GNB3 antibody [EPR8862] (ab154866)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8862] to GNB3
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-GNB3 antibody [EPR8862]
See all GNB3 primary antibodies -
Description
Rabbit monoclonal [EPR8862] to GNB3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HepG2, A673 cell lysate; Human fetal brain tissue lysate; Human fetal lung tissue lysate; Human kidney tissue
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8862 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. -
Sequence similarities
Belongs to the WD repeat G protein beta family.
Contains 7 WD repeats. - Information by UniProt
-
Database links
- Entrez Gene: 2784 Human
- Entrez Gene: 14695 Mouse
- Entrez Gene: 60449 Rat
- Omim: 139130 Human
- SwissProt: P16520 Human
- SwissProt: Q61011 Mouse
- SwissProt: P52287 Rat
- Unigene: 631657 Human
see all -
Alternative names
- G protein beta 3 subunit antibody
- GBB3_HUMAN antibody
- GNB3 antibody
see all
Images
-
All lanes : Anti-GNB3 antibody [EPR8862] (ab154866) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : A673 cell lysate
Lane 4 : Human fetal lung tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling GNB3 with ab154866 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human normal tonsil tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human normal heart tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human retina tissue using ab154866 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab154866 has been referenced in 2 publications.
- Wang Q et al. Identification of RyR2-PBmice and the effects of transposon insertional mutagenesis of the RyR2 gene on cardiac function in mice. PeerJ 7:e6942 (2019). PubMed: 31143551
- McGlinchey JC et al. Correction of the Pathogenic Alternative Splicing, Caused by the Common GNB3 c.825C>T Allele, Using a Novel, Antisense Morpholino. Nucleic Acid Ther 26:257-65 (2016). PubMed: 27028457