Anti-ATG12 antibody (ab155589)
Key features and details
- Rabbit polyclonal to ATG12
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-ATG12 antibody
See all ATG12 primary antibodies -
Description
Rabbit polyclonal to ATG12 -
Host species
Rabbit -
Specificity
ab155589 detects free ATG12 and ATG12 conjugated to Atg5. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human ATG12 aa 1-187. The exact sequence is proprietary.
-
Positive control
- 293T, A431, NIH3T3 and Rat brain whole cell lysates; Human U87 xenograft tissue; HeLa cells untreated or treated with Tunicamycin.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 15 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
|
ICC/IF |
1/100 - 1/1000.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 15 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
-
Function
Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the ATG12 family. -
Domain
Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 9140 Human
- Entrez Gene: 67526 Mouse
- Entrez Gene: 361321 Rat
- Omim: 609608 Human
- SwissProt: O94817 Human
- SwissProt: Q9CQY1 Mouse
- SwissProt: Q2TBJ5 Rat
- Unigene: 264482 Human
see all -
Alternative names
- Apg12 antibody
- APG12-like antibody
- APG12L antibody
see all
Images
-
All lanes : Anti-ATG12 antibody (ab155589) at 1/500 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : ATG12 knockout THP-1 cell lysate
Lane 3 : HCT 116 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-ATG12 antibody staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab155589 was shown to bind specifically to ATG12. A band likely to be the unfunctional complex with ATG5 was observed at 52 kDa in wild-type THP-1 cell lysates with no signal observed at this size in Atg12 knockout cell line ab277831 (knockout cell lysate ab278183) - unconjugated functional form not observed at 15 kD. To generate this image, wild-type and Atg12 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
-
Anti-ATG12 antibody (ab155589) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg
Predicted band size: 15 kDa
12% SDS PAGE -
Anti-ATG12 antibody (ab155589) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg
Predicted band size: 15 kDa
12% SDS PAGE -
All lanes : Anti-ATG12 antibody (ab155589) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 15 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded U87 (human) xenograft tissue labeling ATG12 with ab155589 at 1/500 dilution.
-
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling ATG12 with ab155589 at 1/500 dilution. Lower panels co-stained with Hoechst 33342.
Cells were fixed in 4% paraformaldehyde for 15 minutes.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (27)
ab155589 has been referenced in 27 publications.
- Zhang H et al. Identification of Key Genes and Potential Mechanisms Based on the Autophagy Regulatory Network in Osteoclasts Using a Murine Osteoarthritis Model. J Inflamm Res 15:2333-2347 (2022). PubMed: 35437349
- Zhu T et al. ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC. Front Oncol 12:929838 (2022). PubMed: 36059676
- Li H et al. Destabilization of TP53 by USP10 is essential for neonatal autophagy and survival. Cell Rep 41:111435 (2022). PubMed: 36198274
- Xu Y et al. Sp1 Targeted PARP1 Inhibition Protects Cardiomyocytes From Myocardial Ischemia-Reperfusion Injury via Downregulation of Autophagy. Front Cell Dev Biol 9:621906 (2021). PubMed: 34124031
- Zhang J et al. PSPH induces cell autophagy and promotes cell proliferation and invasion in the hepatocellular carcinoma cell line Huh7 via the AMPK/mTOR/ULK1 signaling pathway. Cell Biol Int 45:305-319 (2021). PubMed: 33079432