Recombinant Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10470(B)] to DNA polymerase mu
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DNA polymerase mu antibody [EPR10470(B)]
See all DNA polymerase mu primary antibodies -
Description
Rabbit monoclonal [EPR10470(B)] to DNA polymerase mu -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T, HeLa, Daudi and Jurkat cell lysates. IP: Jurkat cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10470(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 55 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 55 kDa. |
Target
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Function
Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination. -
Tissue specificity
Expressed in a number of tissues. Abundant in thymus. -
Sequence similarities
Belongs to the DNA polymerase type-X family.
Contains 1 BRCT domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 27434 Human
- Entrez Gene: 54125 Mouse
- Entrez Gene: 289757 Rat
- Omim: 606344 Human
- SwissProt: Q9NP87 Human
- SwissProt: Q9JIW4 Mouse
- Unigene: 596982 Human
- Unigene: 260194 Mouse
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Alternative names
- DNA-directed DNA polymerase mu antibody
- DNA-directed DNA/RNA polymerase mu antibody
- DPOLM_HUMAN antibody
see all
Images
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All lanes : Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : POLM knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 55 kDaLanes 1-4: Merged signal (red and green). Green - ab157465 observed at 52 kDa. Red - loading control ab8245 observed at 37 kDa.
ab157465 Anti-DNA polymerase mu antibody [EPR10470(B)] was shown to specifically react with POLM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265557 (knockout cell lysate ab258127) was used. Wild-type and POLM knockout samples were subjected to SDS-PAGE. ab157465 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa -
Anti-DNA polymerase mu antibody [EPR10470(B)] (ab157465) at 1/1000 dilution + immunoprecipitation pellet from Jurkat cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab157465 has been referenced in 2 publications.
- Schimmel J et al. Mutational signatures of non-homologous and polymerase theta-mediated end-joining in embryonic stem cells. EMBO J N/A:N/A (2017). WB ; Mouse . PubMed: 29079701
- Gassman NR et al. Hyperactivation of PARP triggers nonhomologous end-joining in repair-deficient mouse fibroblasts. PLoS One 7:e49301 (2012). PubMed: 23145148