Recombinant Anti-Fbx32 antibody [EPR9148(2)] (ab168372)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9148(2)] to Fbx32
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Fbx32 antibody [EPR9148(2)]
See all Fbx32 primary antibodies -
Description
Rabbit monoclonal [EPR9148(2)] to Fbx32 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Fbx32.
Database link: Q969P5 -
Positive control
- Human skeletal muscle, mouse heart, mouse muscle and rat muscle lysates; A673 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9148(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
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ICC/IF |
1/200.
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). |
ICC/IF
1/200. |
Target
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Function
Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. -
Tissue specificity
Specifically expressed in cardiac and skeletal muscle. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 F-box domain. - Information by UniProt
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Database links
- Entrez Gene: 114907 Human
- Entrez Gene: 67731 Mouse
- Entrez Gene: 171043 Rat
- Omim: 606604 Human
- SwissProt: Q969P5 Human
- SwissProt: Q9CPU7 Mouse
- SwissProt: Q91Z62 Rat
- Unigene: 403933 Human
see all -
Alternative names
- 4833442G10Rik antibody
- AI430017 antibody
- Atrogin 1 antibody
see all
Images
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All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified)
Lane 1 : Mouse muscle
Lane 2 : Rat muscle
Lysates/proteins at 20 µg per lane.
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunofluorescent staining of A673 cells (fixed in 4% PFA) using purified ab168372 at a dilution of 1/200. An Alexa Fluor® 555 goat anti-rabbit (ab150082) was used at a dilution of 1/500 and the cells were counter-stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, purified ab168372 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/400.
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Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (Purified) + Human skeletal muscle at 10 µg
Secondary
HRP anti-rabbit IgG (specific to non-reduced IgG) at 1/1000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunofluorescent analysis of A673 cells labeling Fbx32 with ab168372 at 1/250 dilution.
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All lanes : Anti-Fbx32 antibody [EPR9148(2)] (ab168372) at 1/1000 dilution (unpurified)
Lane 1 : mouse heart lysate
Lane 2 : rat muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (82)
ab168372 has been referenced in 82 publications.
- Chen L et al. CPNE1 regulates myogenesis through the PERK-eIF2α pathway mediated by endoplasmic reticulum stress. Cell Tissue Res 391:545-560 (2023). PubMed: 36525128
- Miyazaki M et al. Supplementing cultured human myotubes with hibernating bear serum results in increased protein content by modulating Akt/FOXO3a signaling. PLoS One 17:e0263085 (2022). PubMed: 35077510
- Gu L et al. TLR13 contributes to skeletal muscle atrophy by increasing insulin resistance in chronic kidney disease. Cell Prolif 55:e13181 (2022). PubMed: 35088922
- Chi MY et al. Silibinin Alleviates Muscle Atrophy Caused by Oxidative Stress Induced by Cisplatin through ERK/FoxO and JNK/FoxO Pathways. Oxid Med Cell Longev 2022:5694223 (2022). PubMed: 35096269
- Li Q et al. Paeoniflorin Ameliorates Skeletal Muscle Atrophy in Chronic Kidney Disease via AMPK/SIRT1/PGC-1α-Mediated Oxidative Stress and Mitochondrial Dysfunction. Front Pharmacol 13:859723 (2022). PubMed: 35370668