Recombinant Anti-CDKAL1 antibody [EPR11621] (ab168390)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11621] to CDKAL1
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CDKAL1 antibody [EPR11621]
See all CDKAL1 primary antibodies -
Description
Rabbit monoclonal [EPR11621] to CDKAL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human CDKAL1 aa 1-200. The exact sequence is proprietary.
Database link: Q5VV42 -
Positive control
- HepG2, Raji, MDA-MB-435 and HeLa cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11621 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168390 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 65 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 65 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine. -
Tissue specificity
Expressed in pancreatic islets. -
Involvement in disease
Genetic variations in CDKAL1 are a cause of susceptibility to diabetes mellitus, non-insulin-dependent (NIDDM) [MIM:125853]. A multifactorial disorder of glucose homeostasis caused by a lack of sensitivity to the body's own insulin. Affected individuals usually have an obese body habitus and manifestations of a metabolic syndrome characterized by diabetes, insulin resistance, hypertension and hypertriglyceridemia. The disease results in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Belongs to the methylthiotransferase family. CDKAL1 subfamily.
Contains 1 MTTase N-terminal domain.
Contains 1 TRAM domain. -
Cellular localization
Endoplasmic reticulum membrane. Is a tail-anchored protein that exploits the TCR40 pathway for insertion into the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 54901 Human
- Entrez Gene: 68916 Mouse
- Omim: 611259 Human
- SwissProt: Q5VV42 Human
- SwissProt: Q91WE6 Mouse
- Unigene: 657604 Human
- Unigene: 212227 Mouse
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Alternative names
- CDK5 regulatory subunit associated protein 1 like 1 antibody
- CDK5 regulatory subunit-associated protein 1-like 1 antibody
- CDKAL_HUMAN antibody
see all
Images
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All lanes : Anti-CDKAL1 antibody [EPR11621] (ab168390) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Raji cell lysate
Lane 3 : MDA-MB-435 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 65 kDa -
Detection of CDKAL1 by Western Blot of Immunprecipitate. HepG2 cell lysate immunoprecipitated using ab168390 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab168390 has not yet been referenced specifically in any publications.