Recombinant Anti-RRM2 antibody [EPR11820] (ab172476)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11820] to RRM2
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RRM2 antibody [EPR11820]
See all RRM2 primary antibodies -
Description
Rabbit monoclonal [EPR11820] to RRM2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human RRM2. The exact sequence is proprietary.
Database link: P31350 -
Positive control
- WB: HeLa, HepG2, K562, Jurkat. ICC/IF: HeLa. IHC: Human cerebrum, colon carcinoma, human stomach. IP: Hela
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11820 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
IP
1/10 - 1/100. |
Target
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Function
Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling. -
Pathway
Genetic information processing; DNA replication. -
Sequence similarities
Belongs to the ribonucleoside diphosphate reductase small chain family. -
Post-translational
modificationsPhosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6241 Human
- Omim: 180390 Human
- SwissProt: P31350 Human
- Unigene: 226390 Human
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Alternative names
- cb111 antibody
- chunp6884 antibody
- etID309896.20 antibody
see all
Images
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Immunohistochemical analysis of Paraffin-embedded sections Human Colon carcinoma tissue labelling RRM2 with ab172476 at 1/1000 dilution, followed by LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use). Positive staining on Human Colon carcinoma is observed. The section was incubated with ab172476 for 30 mins at room temperature. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use).
Positive staining on human colon carcinoma. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.
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Immunohistochemical analysis of Paraffin-embedded sections Human Stomach tissue labelling RRM2 with ab172476 at 1/1000 dilution, followed by LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use).Human stomach staining on Human Stomach is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use).
Positive staining in human stomach. The section was incubated with ab172476 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins.
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Immunohistochemical analysis of Paraffin-embedded sections Human Cerebrum tissue labelling RRM2 with ab172476 at 1/1000 dilution, followed by LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use). Negative/low expression control: no staining in human cerebrum (PMID: 30745840). The section was incubated with ab172476 for 30 mins at room temperature. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is LeicaDS9800 (Bond® Polymer Refine Detection)(Ready to use). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins
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All lanes : Anti-RRM2 antibody [EPR11820] (ab172476) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa -
Immunofluorescent analysis of HeLa cells labeling RRM2 with ab172476 at 1/100 dilution (red). DAPI nuclear staining (blue).
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Western blot analysis on immunoprecipitation pellet from HeLa cell lysate (lane 1) labeling RRM2 with ab172476 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab172476 has been referenced in 17 publications.
- Song C et al. Polyphyllin VII suppresses cell proliferation, the cell cycle and cell migration in colorectal cancer. Oncol Lett 21:25 (2021). PubMed: 33240431
- Tian Q et al. Development and Validation of a Ferroptosis-Related Gene Signature for Overall Survival Prediction in Lung Adenocarcinoma. Front Cell Dev Biol 9:684259 (2021). PubMed: 34307361
- Cao C et al. Expression of CHPF modulates cell proliferation and invasion in lung cancer. Braz J Med Biol Res 53:e9021 (2020). PubMed: 32348423
- Yu L et al. Longitudinal Analysis of Gene Expression Changes During Cervical Carcinogenesis Reveals Potential Therapeutic Targets. Evol Bioinform Online 16:1176934320920574 (2020). PubMed: 32489245
- Li S et al. MicroRNA-4500 Inhibits Migration, Invasion, and Angiogenesis of Breast Cancer Cells via RRM2-Dependent MAPK Signaling Pathway. Mol Ther Nucleic Acids 21:278-289 (2020). PubMed: 32615527