Recombinant Anti-SF3B1 antibody [EPR11986] (ab172634)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11986] to SF3B1
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SF3B1 antibody [EPR11986]
See all SF3B1 primary antibodies -
Description
Rabbit monoclonal [EPR11986] to SF3B1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SF3B1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O75533 -
Positive control
- WB: HeLa, Jurkat, K562 and Ramos cell lysates. IHC-P: Human colon and glioma tissues. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11986 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/2000.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 146 kDa.
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ICC/IF |
1/250 - 1/500.
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Notes |
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Flow Cyt (Intra)
1/2000. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 146 kDa. |
ICC/IF
1/250 - 1/500. |
Target
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Relevance
SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. -
Cellular localization
Nucleus speckle. Note=During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm. -
Database links
- Entrez Gene: 23451 Human
- Entrez Gene: 81898 Mouse
- Entrez Gene: 84486 Rat
- Omim: 605590 Human
- SwissProt: O75533 Human
- SwissProt: Q99NB9 Mouse
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Alternative names
- Hsh 155 antibody
- MDS antibody
- Pre mRNA processing 10 antibody
see all
Images
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All lanes : Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : Jurkat lysate
Lane 3 : K562 lysate
Lane 4 : Ramos lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 146 kDa -
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human colon tissue labeling SF3B1 with ab172634 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of HeLa cells labeling SF3B1 with ab172634 at 1/250 dilution.
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SF3B1 (red) with ab172634 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue labeling SF3B1 with ab172634 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab172634 has been referenced in 13 publications.
- Fuentes-Fayos AC et al. SF3B1 inhibition disrupts malignancy and prolongs survival in glioblastoma patients through BCL2L1 splicing and mTOR/ß-catenin pathways imbalances. J Exp Clin Cancer Res 41:39 (2022). PubMed: 35086552
- Simmler P et al. SF3B1 facilitates HIF1-signaling and promotes malignancy in pancreatic cancer. Cell Rep 40:111266 (2022). PubMed: 36001976
- Rouf Banday A et al. Targeting natural splicing plasticity of APOBEC3B restricts its expression and mutagenic activity. Commun Biol 4:386 (2021). PubMed: 33753867
- Yang JY et al. SF3B1 mutation in pancreatic cancer contributes to aerobic glycolysis and tumor growth through a PP2A-c-Myc axis. Mol Oncol 15:3076-3090 (2021). PubMed: 33932092
- Alors-Perez E et al. Dysregulated splicing factor SF3B1 unveils a dual therapeutic vulnerability to target pancreatic cancer cells and cancer stem cells with an anti-splicing drug. J Exp Clin Cancer Res 40:382 (2021). PubMed: 34857016