Recombinant Anti-MEIS2 antibody [EPR12100] (ab174270)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12100] to MEIS2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MEIS2 antibody [EPR12100]
See all MEIS2 primary antibodies -
Description
Rabbit monoclonal [EPR12100] to MEIS2 -
Host species
Rabbit -
Specificity
The immunogen of ab174270 is, according to BLAST results, highly conserved (93%) with human MEIS1. According to this, the antibody is very likely to cross-react with MEIS1 but this has, to our knowledge, not been experimentally tested so far. -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human MEIS2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O14770 -
Positive control
- Fetal thymus, HeLa, HepG2 and K562 lysates. Permeabilized K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12100 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
Target
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Tissue specificity
Expressed in various tissues. In hematopoietic tissues, the lymphoid organs express high levels of MEIS2. Also expressed in some regions of the brain, such as the putamen. -
Sequence similarities
Belongs to the TALE/MEIS homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4212 Human
- Entrez Gene: 17536 Mouse
- Entrez Gene: 311311 Rat
- Omim: 601740 Human
- SwissProt: O14770 Human
- SwissProt: P97367 Mouse
- Unigene: 510989 Human
- Unigene: 247566 Mouse
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Alternative names
- Homeobox protein Meis2 antibody
- HsT18361 antibody
- Meis (mouse) homolog 2 antibody
see all
Images
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Intracellular flow cytometric analysis of permeabilized K562 cells usingab174270 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
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All lanes : Anti-MEIS2 antibody [EPR12100] (ab174270) at 1/1000 dilution
Lane 1 : Fetal thymus lysates
Lane 2 : HeLa lysates
Lane 3 : HepG2 lysates
Lane 4 : K562 lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab174270 has not yet been referenced specifically in any publications.