Recombinant Anti-ATRIP antibody [EPR8072(2)] (ab175221)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8072(2)] to ATRIP
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATRIP antibody [EPR8072(2)]
See all ATRIP primary antibodies -
Description
Rabbit monoclonal [EPR8072(2)] to ATRIP -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human ATRIP aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: Q8WXE1 -
Positive control
- PC-3, Hela, MCF-7 cell lysates; MCF-7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8072(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/50 - 1/100.
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|
WB |
1/1000 - 1/5000. Predicted molecular weight: 86 kDa.
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Notes |
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ICC/IF
1/50 - 1/100. |
WB
1/1000 - 1/5000. Predicted molecular weight: 86 kDa. |
Target
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Function
Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the ATRIP family. -
Domain
The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. -
Post-translational
modificationsPhosphorylated by ATR. -
Cellular localization
Nucleus. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 84126 Human
- Entrez Gene: 235610 Mouse
- Entrez Gene: 301014 Rat
- Omim: 606605 Human
- SwissProt: Q8WXE1 Human
- SwissProt: Q8BMG1 Mouse
- Unigene: 694840 Human
- Unigene: 100622 Mouse
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Alternative names
- AGS1 antibody
- ATIP antibody
- ATM and Rad3 related interacting protein antibody
see all
Images
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All lanes : Anti-ATRIP antibody [EPR8072(2)] (ab175221) at 1/1000 dilution
Lane 1 : PC-3 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 86 kDa -
Immunofluorescent staining of MCF-7 cells labeling ATRIP with ab175221 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab175221 has been referenced in 4 publications.
- Hu S et al. LINCS gene expression signature analysis revealed bosutinib as a radiosensitizer of breast cancer cells by targeting eIF4G1. Int J Mol Med 47:N/A (2021). PubMed: 33693953
- Wang J et al. Acetylation of XPF by TIP60 facilitates XPF-ERCC1 complex assembly and activation. Nat Commun 11:786 (2020). PubMed: 32034146
- Huang J et al. Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27:1794-1808.e5 (2019). PubMed: 31067464
- Hopkins J et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet 10:e1004413 (2014). ICC/IF ; Mouse . PubMed: 24992337