Recombinant Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7024(N)] to RPE65 - C-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RPE65 antibody [EPR7024(N)] - C-terminal
See all RPE65 primary antibodies -
Description
Rabbit monoclonal [EPR7024(N)] to RPE65 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Mouse eyeball, rat eyeball and Y79 lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7024(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 61 kDa.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 61 kDa. |
IP
1/10 - 1/100. |
Target
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Function
Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association. -
Tissue specificity
Retinal pigment epithelium specific. -
Involvement in disease
Defects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2) [MIM:204100]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children.
Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20) [MIM:613794]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP20 inheritance is autosomal dominant. -
Sequence similarities
Belongs to the carotenoid oxygenase family. -
Post-translational
modificationsPalmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A). -
Cellular localization
Cytoplasm. Cell membrane. Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated. - Information by UniProt
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Database links
- Entrez Gene: 6121 Human
- Entrez Gene: 19892 Mouse
- Entrez Gene: 89826 Rat
- Omim: 180069 Human
- SwissProt: Q16518 Human
- SwissProt: Q91ZQ5 Mouse
- SwissProt: O70276 Rat
- Unigene: 2133 Human
see all -
Alternative names
- All-trans-retinyl-palmitate hydrolase antibody
- LCA 2 antibody
- LCA2 antibody
see all
Images
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Western blot analysis on immunoprecipitation pellet from mouse eyeball lysate labeling RPE65 with ab175936 at 1/10 dilution
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All lanes : Anti-RPE65 antibody [EPR7024(N)] - C-terminal (ab175936) at 1/1000 dilution
Lane 1 : Mouse eyeball lysate
Lane 2 : Rat eyeball lysate
Lane 3 : Y79 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 61 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab175936 has been referenced in 1 publication.
- Zhou J et al. Correlations between Photodegradation of Bisretinoid Constituents of Retina and Dicarbonyl Adduct Deposition. J Biol Chem 290:27215-27 (2015). WB . PubMed: 26400086