Recombinant Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UMB-8-37-1] to Endothelin A Receptor/ET-A
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1]
See all Endothelin A Receptor/ET-A primary antibodies -
Description
Rabbit monoclonal [UMB-8-37-1] to Endothelin A Receptor/ET-A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse heart and lung tissue lysates; IHC-P: Mouse and rat cardiac muscle and human placenta tissue.
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General notes
This product has switched from a hybridoma to recombinant production method on 9th September 2020.
The epitope recognized by the UMB-8-37-1 monoclonal contains phosphorylation sites that affect signal in Western blot after treatment with endothelin. Phosphatase treatment may be required prior to Western blot in order to improve signal (PubMed ID 25708416).
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team. -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
UMB-8-37-1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 40-70 kDa.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 40-70 kDa. |
Target
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Function
Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 > ET2 >> ET3. -
Tissue specificity
Isoform 1, isoform 3 and isoform 4 are expressed in a variety of tissues, with highest levels in the aorta and cerebellum, followed by lung, atrium and cerebral cortex, lower levels in the placenta, kidney, adrenal gland, duodenum, colon, ventricle and liver but no expression in umbilical vein endothelial cells. Within the placenta, isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in the villi and stem villi vessels. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. Endothelin receptor subfamily. EDNRA sub-subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1909 Human
- Entrez Gene: 13617 Mouse
- Entrez Gene: 24326 Rat
- GenBank: NM_001957 Human
- GenBank: NM_174308 Human
- GenBank: NM_012550 Rat
- Omim: 131243 Human
- SwissProt: P25101 Human
see all -
Alternative names
- Ednra antibody
- EDNRA_HUMAN antibody
- Endothelin 1 receptor precursor antibody
see all
Images
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All lanes : Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40-70 kDa
Observed band size: 40-70 kDa
Exposure time: 26 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The observed molecular weight is consistent with what has been described in the literature (PMID: 25708416).
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Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).
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Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).
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Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Endothelin A Receptor/ET-A with ab178454 at 1/100 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using Tris/EDTA buffer (pH 9.0).
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All lanes : Anti-Endothelin A Receptor/ET-A antibody [UMB-8-37-1] (ab178454) at 1/1000 dilution
Lane 1 : HEK-293 (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293 transfected with Endothelin A Receptor/ET-A (WT) expression vector containing a myc-His-tag® , whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40-70 kDa
Observed band size: 40-80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical localization of ETA in human normal lymph node (I) and in neoplastic tissues (A–H) using ab178454. For more protocol information please see PMID: 25708416.
Image generated from hybridoma version of ab178454
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab178454 has been referenced in 9 publications.
- Cai C et al. Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway. Redox Biol 52:102288 (2022). PubMed: 35325804
- Yu Q et al. Sorafenib not only impairs endothelium-dependent relaxation but also promotes vasoconstriction through the upregulation of vasoconstrictive endothelin type B receptors. Toxicol Appl Pharmacol 414:115420 (2021). PubMed: 33503445
- Puig-Domingo M et al. Molecular profiling for acromegaly treatment: a validation study. Endocr Relat Cancer 27:375-389 (2020). PubMed: 32302973
- Kimm MA et al. Targeting Endothelin Receptors in a Murine Model of Myocardial Infarction Using a Small Molecular Fluorescent Probe. Mol Pharm 17:109-117 (2020). PubMed: 31816245
- Stölting M et al. Monitoring Endothelin-A Receptor Expression during the Progression of Atherosclerosis. Biomedicines 8:N/A (2020). PubMed: 33255872
- Cianfrocca R et al. Blocking endothelin-1-receptor/ß-catenin circuit sensitizes to chemotherapy in colorectal cancer. Cell Death Differ 24:1811-1820 (2017). WB ; Human . PubMed: 28708138
- Kaemmerer D et al. Evaluation of somatostatin, CXCR4 chemokine and endothelin A receptor expression in a large set of paragangliomas. Oncotarget 8:89958-89969 (2017). IHC-P ; Human . PubMed: 29163802
- Mahmoud AM et al. Hyperinsulinemia augments endothelin-1 protein expression and impairs vasodilation of human skeletal muscle arterioles. Physiol Rep 4:N/A (2016). WB ; Human . PubMed: 27796268
- Lupp A et al. Reassessment of endothelin receptor A expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-8. Peptides 66:19-25 (2015). IHC-P, ICC, WB ; Mouse, Human . PubMed: 25708416