Recombinant Anti-Thyroid Hormone Receptor alpha antibody [EPR7862] (ab179438)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7862] to Thyroid Hormone Receptor alpha
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Thyroid Hormone Receptor alpha antibody [EPR7862]
See all Thyroid Hormone Receptor alpha primary antibodies -
Description
Rabbit monoclonal [EPR7862] to Thyroid Hormone Receptor alpha -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3, MEF, and D1.1 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7862 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 55 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 55 kDa. |
Target
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Function
Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine. -
Involvement in disease
Hypothyroidism, congenital, non-goitrous, 6 -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7067 Human
- Entrez Gene: 21833 Mouse
- Omim: 190120 Human
- SwissProt: P10827 Human
- SwissProt: P63058 Mouse
- Unigene: 724 Human
- Unigene: 265917 Mouse
- Unigene: 442648 Mouse
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Alternative names
- AR7 antibody
- c-erbA-1 antibody
- C-erbA-alpha antibody
see all
Images
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All lanes : Anti-Thyroid Hormone Receptor alpha antibody [EPR7862] (ab179438) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 2 : MEF (mouse embryo fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 180 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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Anti-Thyroid Hormone Receptor alpha antibody [EPR7862] (ab179438) at 1/1000 dilution + D1.1 (human T lymphoblast), whole cell lysate at 50 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
Exposure time: 59 secondsBlocking and dilution buffer: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab179438 has not yet been referenced specifically in any publications.