Recombinant Anti-GIV antibody [EPR18433] (ab179481)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18433] to GIV
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GIV antibody [EPR18433]
See all GIV primary antibodies -
Description
Rabbit monoclonal [EPR18433] to GIV -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HCT 116, HeLa, C6, RAW 264.7 and PC-12 whole cell lysates; Human fetal kidney, mouse brain and rat brain lysates. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18433 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/150.
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WB |
1/1000. Detects a band of approximately 208-216 kDa (predicted molecular weight: 216 kDa).
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ICC/IF |
1/2000.
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IP |
1/50.
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Notes |
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Flow Cyt (Intra)
1/150. |
WB
1/1000. Detects a band of approximately 208-216 kDa (predicted molecular weight: 216 kDa). |
ICC/IF
1/2000. |
IP
1/50. |
Target
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Function
Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. -
Tissue specificity
Expressed ubiquitously. -
Sequence similarities
Belongs to the CCDC88 family. -
Post-translational
modificationsPhosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration. -
Cellular localization
Membrane. Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Cell projection > lamellipodium. Localizes to the cell membrane through interaction with phosphoinositides. - Information by UniProt
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Database links
- Entrez Gene: 55704 Human
- Entrez Gene: 108686 Mouse
- Entrez Gene: 305605 Rat
- Omim: 609736 Human
- SwissProt: Q3V6T2 Human
- SwissProt: Q5SNZ0 Mouse
- Unigene: 292925 Human
- Unigene: 338284 Mouse
see all -
Alternative names
- AKT iphosphorylation enhancer antibody
- Akt phosphorylation enhancer antibody
- APE antibody
see all
Images
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Anti-GIV antibody [EPR18433] (ab179481) at 1/5000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 216 kDa
Observed band size: 208-216 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
Multiple bands represent 5 isoforms ranging from 208 to 216 kDa (PMID: 23195430; PMID: 22308453).
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GIV was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab179481 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179481 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10µg (Input).
Lane 2: ab179481 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab179481 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds. -
Anti-GIV antibody [EPR18433] (ab179481) at 1/1000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 216 kDa
Observed band size: 208-216 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Multiple bands represent 5 isoforms ranging from 208 to 216 kDa (PMID: 23195430; PMID: 22308453).
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Anti-GIV antibody [EPR18433] (ab179481) at 1/1000 dilution + HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 216 kDa
Observed band size: 208-216 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Multiple bands represent 5 isoforms ranging from 208 to 216 kDa (PMID: 23195430; PMID: 22308453).
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All lanes : Anti-GIV antibody [EPR18433] (ab179481) at 1/5000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 216 kDa
Observed band size: 208-216 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 30 seconds; Lane 2: 3 minutes.
Based on UniProt annotation, mouse GIV has 3 isoforms (215, 212, 205 kDa).
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All lanes : Anti-GIV antibody [EPR18433] (ab179481) at 1/5000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 216 kDa
Observed band size: 208-216 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Based on UniProt annotation, mouse GIV has 3 isoforms (215, 212, 205KD).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GIV with ab179481 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab179481 at 1/2000 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution. -
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling GIV with ab179481 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab179481 at 1/2000 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution. -
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling GIV with ab179481 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (8)
ab179481 has been referenced in 8 publications.
- Lan Y et al. SIRT1-induced deacetylation of Akt expedites platelet phagocytosis and delays HEMEC aging. Mol Ther Nucleic Acids 23:1323-1333 (2021). PubMed: 33717652
- Dang J et al. Roles of metformin-mediated girdin expression in metastasis of epithelial ovarian cancer. Cell Biol Int 45:1030-1037 (2021). PubMed: 33404163
- Hao N et al. Phosphodiesterase 3A Represents a Therapeutic Target that Drives Stem Cell-like Property and Metastasis in Breast Cancer. Mol Cancer Ther 19:868-881 (2020). PubMed: 31871268
- Wang W et al. Girdin interaction with vimentin induces EMT and promotes the growth and metastasis of pancreatic ductal adenocarcinoma. Oncol Rep 44:637-649 (2020). PubMed: 32467989
- Wang S et al. Girdin regulates the proliferation and apoptosis of pancreatic cancer cells via the PI3K/Akt signalling pathway. Oncol Rep 40:599-608 (2018). PubMed: 29901184
- Lan Y et al. Engulfment of platelets delays endothelial cell aging via girdin and its phosphorylation. Int J Mol Med 42:988-997 (2018). PubMed: 29786109
- Lu J et al. Silencing of Girdin suppresses the malignant behavior of colorectal carcinoma cells. Oncol Rep 40:887-894 (2018). PubMed: 29989653
- Yu L et al. Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism. J Exp Clin Cancer Res 36:110 (2017). PubMed: 28810896