Recombinant Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (ab180506)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14505(B)] to eIF4A1 - N-terminal
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal
See all eIF4A1 primary antibodies -
Description
Rabbit monoclonal [EPR14505(B)] to eIF4A1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, HepG2, MCF7, K562 cell lysates. MCF7 and K562 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14505(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
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ICC/IF |
1/250.
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Notes |
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Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa. |
ICC/IF
1/250. |
Target
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Function
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. -
Sequence similarities
Belongs to the DEAD box helicase family. eIF4A subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. - Information by UniProt
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Database links
- Entrez Gene: 1973 Human
- Entrez Gene: 13681 Mouse
- Entrez Gene: 287436 Rat
- Omim: 602641 Human
- SwissProt: P60842 Human
- SwissProt: P60843 Mouse
- Unigene: 129673 Human
- Unigene: 279821 Mouse
see all -
Alternative names
- ATP-dependent RNA helicase eIF4A-1 antibody
- DDX2 antibody
- DDX2A antibody
see all
Images
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All lanes : Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (ab180506) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDaBlocking buffer: 5% NFDM/TBST
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All lanes : Anti-eIF4A1 antibody [EPR14505(B)] - N-terminal (ab180506) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa -
Immunofluorescence analysis of MCF-7 cells labeling eIF4A1 (green) with ab180506 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).
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Intracellular Flow Cytometry analysis of eIF4A1 expression in K562 cells using ab180506 at 1/50 dilution (red) and a rabbit IgG as negative control (green). A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody at 1/75 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab180506 has not yet been referenced specifically in any publications.