Recombinant Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13657] to Mad2L2/REV7
- Suitable for: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Mad2L2/REV7 antibody [EPR13657]
See all Mad2L2/REV7 primary antibodies -
Description
Rabbit monoclonal [EPR13657] to Mad2L2/REV7 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: K562, SW480, Hela, Molt-4 cell lysates. IHC-P: Human ovarian carcinoma tissue. ICC/IF: Hela cells. Flow Cyt (intra): K562 cells IP: HeLa cell lysates
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13657 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Alexa Fluor® 647 Anti-Mad2L2/REV7 antibody [EPR13657] (ab209029)
- Alexa Fluor® 594 Anti-Mad2L2/REV7 antibody [EPR13657] (ab210455)
- Alexa Fluor® 555 Anti-Mad2L2/REV7 antibody [EPR13657] (ab210653)
- Anti-Mad2L2/REV7 antibody [EPR13657] - BSA and Azide free (ab232620)
- Alexa Fluor® 568 Anti-Mad2L2/REV7 antibody [EPR13657] (ab312441)
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/70.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IP |
1/40.
|
|
ICC/IF |
1/100.
|
|
IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB | (3) |
1/1000. Predicted molecular weight: 24 kDa.
|
Notes |
---|
Flow Cyt (Intra)
1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/40. |
ICC/IF
1/100. |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 24 kDa. |
Target
-
Function
Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Contains 1 HORMA domain. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 10459 Human
- Entrez Gene: 71890 Mouse
- Entrez Gene: 313702 Rat
- Omim: 604094 Human
- SwissProt: Q9UI95 Human
- SwissProt: Q9D752 Mouse
- SwissProt: D3Z8D9 Rat
- Unigene: 19400 Human
see all -
Alternative names
- Homolog of REV7 S cerevisiae antibody
- hREV7 antibody
- MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
see all
Images
-
All lanes : Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : SW480 cell lysate
Lane 3 : Hela cell lysate
Lane 4 : Molt-4 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 24 kDa -
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Mad2L2/REV7 with ab129199 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
Immunoprecipitation of HeLa cell lysate labeling Mad2L2/REV7 using ab180579 at 1/40 dilution (lane 1) or negative control (lane 2) followed by anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
-
Intracellular flow cytometric analysis of paraformadehyde fixed K562 cells labeling Mad2L2/REV7 with ab180579 at 1/70 dilution andGoat anti rabbit IgG (FITC) at 1/50.
-
Immunohistochemical staining of paraffin-embedded human ovarian carcinoma tissue labeling Mad2L2/REV7 using ab180579 at a 1/50 dilution and ImmunoHistoprobe HRP Polymer for Rabbit IgG. Counterstained with hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (21)
ab180579 has been referenced in 21 publications.
- Schubert L et al. SCAI promotes error-free repair of DNA interstrand crosslinks via the Fanconi anemia pathway. EMBO Rep 23:e53639 (2022). PubMed: 35156773
- Bayley R et al. H3K4 methylation by SETD1A/BOD1L facilitates RIF1-dependent NHEJ. Mol Cell 82:1924-1939.e10 (2022). PubMed: 35439434
- Tsaridou S et al. 53BP1-mediated recruitment of RASSF1A to ribosomal DNA breaks promotes local ATM signaling. EMBO Rep 23:e54483 (2022). PubMed: 35758159
- Cho YE et al. Role of the WNT/β-catenin/ZKSCAN3 Pathway in Regulating Chromosomal Instability in Colon Cancer Cell lines and Tissues. Int J Mol Sci 23:N/A (2022). PubMed: 36012568
- Paniagua I et al. MAD2L2 promotes replication fork protection and recovery in a shieldin-independent and REV3L-dependent manner. Nat Commun 13:5167 (2022). PubMed: 36075897