Recombinant Anti-TMEM161A antibody [EPR14369] (ab180954)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14369] to TMEM161A
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TMEM161A antibody [EPR14369]
See all TMEM161A primary antibodies -
Description
Rabbit monoclonal [EPR14369] to TMEM161A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HT 1080, HepG2 and Human fetal liver lysates; Human lung adenocarcinoma tissue; Human infiltrating duct carcinoma of breast tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14369 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
May play a role in protection against oxidative stress. Overexpression leads to reduced levels of oxidant-induced DNA damage and apoptosis. -
Sequence similarities
Belongs to the TMEM161 family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 54929 Human
- SwissProt: Q9NX61 Human
- Unigene: 631629 Human
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Alternative names
- Adaptive response to oxidative stress protein 29 antibody
- AROS-29 antibody
- T161A_HUMAN antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling TMEM161A with ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling TMEM161A with ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-TMEM161A antibody [EPR14369] (ab180954) at 1/20000 dilution + Human fetal liver tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted? -
All lanes : Anti-TMEM161A antibody [EPR14369] (ab180954) at 1/100000 dilution
Lane 1 : HT1080 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab180954 has been referenced in 1 publication.
- Chiou SH et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity 54:586-602.e8 (2021). PubMed: 33691136