Recombinant Anti-MMP23 antibody [EPR7769] (ab181118)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7769] to MMP23
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MMP23 antibody [EPR7769]
See all MMP23 primary antibodies -
Description
Rabbit monoclonal [EPR7769] to MMP23 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain tissue lysate, Calu 3 cell lysate, SK OV 3 cell lysate, Human testis tissue, A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7769 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 44 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 44 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
Target
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Function
Protease. May regulate the surface expression of some potassum channels by retaining them in the endoplasmic reticulum. -
Tissue specificity
Predominantly expressed in ovary, testis and prostate. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The toxin-like domain associates with, and blocks several potassium channels in the nanomolar to low micromolar range. The relative affinity is Kv1.6 > Kv1.3 > Kv1.1 = Kv3.2 > Kv1.4. -
Post-translational
modificationsN-glycosylated.
Proteolytic cleavage might yield an active form. -
Cellular localization
Endoplasmic reticulum membrane. Membrane. A secreted form produced by proteolytic cleavage may also exist. - Information by UniProt
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Database links
- Entrez Gene: 8510 Human
- Entrez Gene: 8511 Human
- Entrez Gene: 26561 Mouse
- Entrez Gene: 94339 Rat
- Omim: 603320 Human
- Omim: 603321 Human
- SwissProt: O75900 Human
- SwissProt: O88676 Mouse
see all -
Alternative names
- Femalysin antibody
- Matrix metallopeptidase 21 antibody
- Matrix metallopeptidase 23A (pseudogene) antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded Human testis tissue labeling MMP23 with ab181118 at 1/250.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling MMP23 with ab181118 at 1/100 followed by fluorescence labelled Goat anti-rabbit IgG at 1/200 and counterstained with Dapi.
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All lanes : Anti-MMP23 antibody [EPR7769] (ab181118) at 1/1000 dilution
Lane 1 : Calu 3 cell lysate
Lane 2 : SK OV 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted? -
Anti-MMP23 antibody [EPR7769] (ab181118) at 1/1000 dilution + Human fetal brain tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 44 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181118 has not yet been referenced specifically in any publications.