Recombinant Anti-MRRF antibody [EPR14140(B)] (ab181223)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14140(B)] to MRRF
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MRRF antibody [EPR14140(B)]
See all MRRF primary antibodies -
Description
Rabbit monoclonal [EPR14140(B)] to MRRF -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human cardiac muscle tissue. HeLa, 293, A431 and HepG2 whole cell lysate (ab7900).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14140(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
Target
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Function
Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. May increase the efficiency of translation by recycling ribosomes from one round of translation to another. -
Sequence similarities
Belongs to the RRF family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 92399 Human
- Entrez Gene: 67871 Mouse
- Omim: 604602 Human
- SwissProt: Q96E11 Human
- SwissProt: Q9D6S7 Mouse
- Unigene: 368011 Human
- Unigene: 378232 Mouse
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Alternative names
- 2400002D02Rik antibody
- MGC93722 antibody
- mitochondrial antibody
see all
Images
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All lanes : Anti-MRRF antibody [EPR14140(B)] (ab181223) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lane 3 : Saos-2 (human osteosarcoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
Exposure time: Lane 1-2: 8 seconds, Lane 3: 26 seconds
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Immunohistochemical analysis of paraffin embedded Human cardiac muscle tissue sections labeling MRRF using ab181223 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human kidney tissue sections labeling MRRF using ab181223 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181223 has not yet been referenced specifically in any publications.