Recombinant Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] (ab181251)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13108] to Neuroligin 1 + Neuroligin 4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] -
Description
Rabbit monoclonal [EPR13108] to Neuroligin 1 + Neuroligin 4 -
Host species
Rabbit -
Specificity
The immunogen of this antibody shows 92% sequence homology with the human Neuroligin-1 (UniProt ID Q8N2Q7) and 77% with human Neuroligin-2 (UniProt ID Q8NFZ4) and Neuroligin-3 (UniProt ID Q9NZ94) -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, HeLa and A549 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13108 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
Target
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Cellular localization
Neuroligin 1: Cell membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in synaptic plasma membranes and clustered in synaptic clefts and postsynaptic densities. Neuroligin 4: Cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. -
Database links
- Entrez Gene: 22829 Human
- Entrez Gene: 22871 Human
- Entrez Gene: 57502 Human
- Omim: 300427 Human
- Omim: 400028 Human
- Omim: 600568 Human
- SwissProt: Q8N0W4 Human
- SwissProt: Q8N2Q7 Human
see all
Images
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All lanes : Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] (ab181251) at 1/1000 dilution
Lane 1 : His-tagged human recombinant Neuroligin 1 protein, 10ng
Lane 2 : His-tagged human recombinant Neuroligin 2 protein, 10ng
Lane 3 : His-tagged human recombinant Neuroligin 3 protein, 10ng
Lane 4 : His-tagged human recombinant Neuroligin 4 protein, 10ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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All lanes : Anti-Neuroligin 1 + Neuroligin 4 antibody [EPR13108] (ab181251) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Hela lysate
Lane 3 : A549 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 92 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181251 has been referenced in 2 publications.
- Gatford NJF et al. Neuroligin-3 and Neuroligin-4X form Nanoscopic clusters and regulate growth cone organization and size. Hum Mol Genet N/A:N/A (2021). WB . PubMed: 34542148
- Nguyen TA et al. A Cluster of Autism-Associated Variants on X-Linked NLGN4X Functionally Resemble NLGN4Y. Neuron 106:759-768.e7 (2020). PubMed: 32243781