Recombinant Anti-KLHL6 antibody [EPR13484] (ab182163)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13484] to KLHL6
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-KLHL6 antibody [EPR13484]
See all KLHL6 primary antibodies -
Description
Rabbit monoclonal [EPR13484] to KLHL6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T transfected with KLHL6 expression vector with a N-terminal DDDDK tag, Human tonsil, A549 and Human fetal kidney lysates. IP: A549 cell lysate. ICC/IF: A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13484 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000 - 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
|
ICC/IF |
1/250.
|
|
IP |
1/50.
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Notes |
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WB
1/1000 - 1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa). |
ICC/IF
1/250. |
IP
1/50. |
Target
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Function
Involved in B-lymphocyte antigen receptor signaling and germinal center formation. -
Tissue specificity
Found in germinal center B-cells. -
Sequence similarities
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. - Information by UniProt
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Database links
- Entrez Gene: 89857 Human
- Omim: 614214 Human
- SwissProt: Q8WZ60 Human
- Unigene: 659147 Human
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Alternative names
- Kelch like protein 6 antibody
- kelch-like 6 antibody
- Kelch-like family member 6 antibody
see all
Images
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All lanes : Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control) whole cell lysate
Lane 2 : 293T transfected with KLHL6 expression vector containing a N-terminal DDDDK tag, whole cell lysate
Lane 3 : Human tonsil lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDaBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
Exposure Time: Lane 1-2: 1 second, Lane 3: 40 seconds.
ab181602 was used as loading controls. ab205606 was used as a total protein control.
Tag constructed at C -terminal of overexpression vector may interfere the recognition of this antibody to targeted protein.
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Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab182163 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling KLHL6 with ab182163 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI.
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Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + A549 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa -
Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + Human fetal kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 70 kDa
Observed band size: 70 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab182163 has been referenced in 1 publication.
- Choi J et al. Loss of KLHL6 promotes diffuse large B-cell lymphoma growth and survival by stabilizing the mRNA decay factor roquin2. Nat Cell Biol 20:586-596 (2018). PubMed: 29695787