TripleStain IHC Kit: M&R&G on human tissue (DAB, AP/Red & DAB/Ni) (ab183291)
Overview
-
Product name
TripleStain IHC Kit: M&R&G on human tissue (DAB, AP/Red & DAB/Ni) -
Assay type
Polymer Multiplex -
Product overview
Abcam's TripleStain IHC Kit (ab183291) is designed to be used with user-supplied goat, mouse and rabbit primary antibodies to detect three distinct antigens on a single human tissue or cell samples. This kit has been tested on paraffin embedded tissue; however it may also be used on frozen or freshly prepared monolayer cell smears.
Triple staining uses traditional and non-traditional methods in immunohistostaining to reveal three distinct antigens and their co-expression on a single tissue. Abcam's TripleStain IHC Kit (ab183291) supplies polymer enzyme conjugates: Goat HRP Polymer, Rabbit AP Polymer and Mouse HRP Polymer with three chromogens, DAB (brown); Permanent Red (red); and DAB-Ni (black). Abcam's TripleStain IHC Kit (ab183291) is a non-biotin system, avoiding non-specific binding caused by endogenous biotin. This kit has been optimized to have no cross reaction when detecting three different primary antibodies using our unique blocking system. Simplified steps allow the users to complete triple staining within 3 hours (without antigen retrieval) or 4 hours (with antigen retrieval). The well tested protocol allows the user to permanently mount slides with a coverslip.
-
Tested applications
Suitable for: IHC-P, IHC-Frmore details
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 18 ml 60 ml 6 ml DAB Chromogen (20x) 1 x 6ml 1 x 4ml 1 x 2ml DAB Substrate 1 x 30ml 1 x 120ml 1 x 12ml DAB-Ni Substrate (20x) 1 x 2ml 1 x 6ml 1 x 1ml Goat HRP Polymer 1 x 18ml 1 x 60ml 1 x 6ml Hydrogen Peroxide (20x) 1 x 2ml 1 x 6ml 1 x 1ml Mouse HRP Polymer 1 x 18ml 1 x 60ml 1 x 6ml Nickel Solution (7x) 1 x 6ml 1 x 18ml 1 x 3ml Non-aqueous Mounting Medium 1 x 6ml 0 x 0ml 1 x 6ml Permanent Red Activator (5x) 1 x 7.2ml 1 x 24ml 1 x 3ml Permanent Red Chromogen (100x) 1 x 360µl 1 x 1.2ml 1 x 150µl Permanent Red Substrate 1 x 36ml 1 x 120ml 1 x 15ml Rabbit AP Polymer 1 x 18ml 1 x 60ml 1 x 6ml Triple Stain Blocker 1 x 18ml 1 x 60ml 1 x 6ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
IHC-Fr |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab183291 has not yet been referenced specifically in any publications.