Recombinant Anti-PPIC antibody [EPR15355] - C-terminal (ab184552)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15355] to PPIC - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PPIC antibody [EPR15355] - C-terminal
See all PPIC primary antibodies -
Description
Rabbit monoclonal [EPR15355] to PPIC - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human lung carcinoma tissue; WB: HeLa, U87-MG and Human fetal kidney lysates; U87-MG cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15355 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family.
Contains 1 PPIase cyclophilin-type domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5480 Human
- Omim: 123842 Human
- SwissProt: P45877 Human
- Unigene: 110364 Human
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Alternative names
- Cyclophilin C antibody
- CYPC antibody
- MGC3673 antibody
see all
Images
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All lanes : Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/10000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa -
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PPIC with ab184552 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-PPIC antibody [EPR15355] - C-terminal (ab184552) at 1/50000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab184552 has been referenced in 4 publications.
- Hill SE et al. Chaperoning activity of the cyclophilin family prevents tau aggregation. Protein Sci 31:e4448 (2022). PubMed: 36305768
- de Wilde AH et al. Coronaviruses and arteriviruses display striking differences in their cyclophilin A-dependence during replication in cell culture. Virology 517:148-156 (2018). WB . PubMed: 29249267
- Nguyen D et al. Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import. Nat Commun 9:3765 (2018). PubMed: 30217974
- Andrews MC et al. Systems analysis identifies miR-29b regulation of invasiveness in melanoma. Mol Cancer 15:72 (2016). WB ; Human . PubMed: 27852308