Recombinant Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14676-23] to MAD1L1/MAD1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MAD1L1/MAD1 antibody [EPR14676-23]
See all MAD1L1/MAD1 primary antibodies -
Description
Rabbit monoclonal [EPR14676-23] to MAD1L1/MAD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HepG2, A431, HeLa and MCF7 lysates. Human melanoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14676-23 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Predicted molecular weight: 83 kDa.
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IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Predicted molecular weight: 83 kDa. |
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. -
Tissue specificity
Expressed weakly at G0/G1 and highly at late S and G2/M phase. -
Involvement in disease
Defects in MAD1L1 are involved in the development and/or progression of various types of cancer. -
Sequence similarities
Belongs to the MAD1 family. -
Post-translational
modificationsPhosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore. - Information by UniProt
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Database links
- Entrez Gene: 8379 Human
- Omim: 602686 Human
- SwissProt: Q9Y6D9 Human
- Unigene: 654838 Human
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Alternative names
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
see all
Images
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Immunohistochemical analysis of formalin fixed and paraffin embedded Human melanoma tissue labeling MAD1 with ab184560 at 1/50 dilution, counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-MAD1L1/MAD1 antibody [EPR14676-23] (ab184560) at 1/20000 dilution
Lane 1 : HepG2 lysate
Lane 2 : A431 lysate
Lane 3 : HeLa lysate
Lane 4 : MCF7 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 83 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab184560 has not yet been referenced specifically in any publications.