Recombinant Anti-TEMT antibody [EPR13240-105] (ab184573)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13240-105] to TEMT
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-TEMT antibody [EPR13240-105]
See all TEMT primary antibodies -
Description
Rabbit monoclonal [EPR13240-105] to TEMT -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human adrenal gland, fetal liver and fetal lung lysates; A549 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13240-105 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
Target
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Function
Catalyzes the N-methylation of tryptamine and structurally related compounds. -
Tissue specificity
Widely expressed. The highest levels were in thyroid, adrenal gland, adult and fetal lung. Intermediate levels in heart, placenta, skeletal muscle, testis, small intestine, pancreas, stomach, spinal cord, lymph node and trachea. Very low levels in adult and fetal kidney and liver, in adult spleen, thymus, ovary, colon and bone marrow. Not expressed in peripheral blood leukocytes and brain. -
Sequence similarities
Belongs to the NNMT/PNMT/TEMT family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 11185 Human
- Entrez Gene: 21743 Mouse
- Entrez Gene: 368066 Rat
- Omim: 604854 Human
- SwissProt: O95050 Human
- SwissProt: P40936 Mouse
- Unigene: 632629 Human
- Unigene: 299 Mouse
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Alternative names
- Amine N methyltransferase antibody
- Amine N-methyltransferase antibody
- Aromatic alkylamine N methyltransferase antibody
see all
Images
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All lanes : Anti-TEMT antibody [EPR13240-105] (ab184573) at 1/1000 dilution
Lane 1 : Human adrenal gland lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa -
Anti-TEMT antibody [EPR13240-105] (ab184573) at 1/1000 dilution + A549 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab184573 has not yet been referenced specifically in any publications.