Recombinant Anti-ATP1B2 antibody [EPR15460(B)] (ab185210)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15460(B)] to ATP1B2
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ATP1B2 antibody [EPR15460(B)]
See all ATP1B2 primary antibodies -
Description
Rabbit monoclonal [EPR15460(B)] to ATP1B2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Y79 and Human cerebellum lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15460(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab185210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 33 kDa).
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IP |
1/50 - 1/70.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 33 kDa). |
IP
1/50 - 1/70. |
Target
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Function
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-2 subunit is not known. -
Sequence similarities
Belongs to the X(+)/potassium ATPases subunit beta family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 482 Human
- Entrez Gene: 11932 Mouse
- Entrez Gene: 24214 Rat
- Omim: 182331 Human
- SwissProt: P14415 Human
- SwissProt: P14231 Mouse
- SwissProt: P13638 Rat
- Unigene: 643540 Human
see all -
Alternative names
- adhesion molecule on glia antibody
- AMOG antibody
- AT1B2 antibody
see all
Images
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Anti-ATP1B2 antibody [EPR15460(B)] (ab185210) at 1/5000 dilution + Y79 (Human retinoblastoma cell line) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa -
Anti-ATP1B2 antibody [EPR15460(B)] (ab185210) at 1/20000 dilution + Human cerebellum lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa -
Western blot analysis of immunoprecipitation pellet from Human cerebellum lysate immunoprecipitated using ab185210 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (6)
ab185210 has been referenced in 6 publications.
- Bai H et al. The Na+, K+-ATPase ß1 subunit regulates epithelial tight junctions via MRCKa. JCI Insight 6:N/A (2021). PubMed: 33507884
- Zhu L & Liu Z Serum from patients with hypertension promotes endothelial dysfunction to induce trophoblast invasion through the miR-27b-3p/ATPase plasma membrane Ca2+ transporting 1 axis. Mol Med Rep 23:N/A (2021). PubMed: 33760199
- Jannas-Vela S et al. Resting metabolic rate and skeletal muscle SERCA and Na+ /K+ ATPase activities are not affected by fish oil supplementation in healthy older adults. Physiol Rep 8:e14408 (2020). PubMed: 32342642
- Liu S et al. Effects of Vaspin on Insulin Resistance in Rats and Underlying Mechanisms. Sci Rep 8:13542 (2018). PubMed: 30202052
- Ge C et al. The down-regulation of SLC7A11 enhances ROS induced P-gp over-expression and drug resistance in MCF-7 breast cancer cells. Sci Rep 7:3791 (2017). WB ; Human . PubMed: 28630426
- Wang K et al. The role of angiopoietin-2 in nucleus pulposus cells during human intervertebral disc degeneration. Lab Invest 97:971-982 (2017). PubMed: 28394321