Recombinant Anti-EAAT4 antibody [EPR7742] - N-terminal (ab186435)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7742] to EAAT4 - N-terminal
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-EAAT4 antibody [EPR7742] - N-terminal
See all EAAT4 primary antibodies -
Description
Rabbit monoclonal [EPR7742] to EAAT4 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human astrocytoma, U87-MG, A375, PC3, C6, Raw264.7 and NIH 3T3 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7742 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
Target
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Relevance
EAAT4 is expressed predominantly in the cerebellum (Purkinje cells) and weakly in the forebrain, where it transports L-glutamate, L-aspartate and D-aspartate, acting as a symport by cotransporting sodium. EAAT4 may function as a combined transporter and inhibitory glutamate receptor and also functions as an anion-selective ion channel. -
Cellular localization
Membrane; multi-pass membrane protein. -
Database links
- Entrez Gene: 6511 Human
- Entrez Gene: 20513 Mouse
- Entrez Gene: 84012 Rat
- Omim: 600637 Human
- SwissProt: P48664 Human
- SwissProt: O35544 Mouse
- SwissProt: O35921 Rat
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Alternative names
- Excitatory amino acid transporter 4 antibody
- High affinity neuronal glutamate transporter antibody
- MGC33092 antibody
see all
Images
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All lanes : Anti-EAAT4 antibody [EPR7742] - N-terminal (ab186435) at 1/10000 dilution
Lane 1 : Human astrocytoma lysate
Lane 2 : U87-MG cell lysate
Lane 3 : A375 cell lysate
Lane 4 : PC3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa -
All lanes : Anti-EAAT4 antibody [EPR7742] - N-terminal (ab186435) at 1/1000 dilution
Lane 1 : C6 lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 62 kDa
Observed band size: 62 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab186435 has been referenced in 1 publication.
- Meira W et al. A Cystine-Cysteine Intercellular Shuttle Prevents Ferroptosis in xCTKO Pancreatic Ductal Adenocarcinoma Cells. Cancers (Basel) 13:N/A (2021). PubMed: 33801101