Anti-ANKLE1 antibody - C-terminal (ab189990)
Key features and details
- Chicken polyclonal to ANKLE1 - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-ANKLE1 antibody - C-terminal
See all ANKLE1 primary antibodies -
Description
Chicken polyclonal to ANKLE1 - C-terminal -
Host species
Chicken -
Specificity
ab189990 is predicted to not cross-react with ANKLE2. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human ANKLE1 (C terminal). (15 amino acid peptide near the carboxy terminus). NP_689576.4.
Database link: Q8NAG6 -
Positive control
- Human skin tissue; 293 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 67 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 67 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Sequence similarities
Contains 3 ANK repeats.
Contains 1 LEM domain. - Information by UniProt
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Database links
- Entrez Gene: 126549 Human
- Entrez Gene: 234396 Mouse
- Entrez Gene: 361122 Rat
- SwissProt: Q8NAG6 Human
- Unigene: 721610 Human
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Alternative names
- 8430438L13Rik antibody
- ANKL1_HUMAN antibody
- ANKLE1 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling ANKLE1 with ab189990 at 5 µg/ml.
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Lane 1 : Anti-ANKLE1 antibody - C-terminal (ab189990) at 1 µg/ml
Lane 2 : Anti-ANKLE1 antibody - C-terminal (ab189990) at 2 µg/ml
All lanes : 293 cell lysate
Predicted band size: 67 kDa
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab189990 has been referenced in 1 publication.
- Tian J et al. ANKLE1 N6 -Methyladenosine-related variant is associated with colorectal cancer risk by maintaining the genomic stability. Int J Cancer 146:3281-3293 (2020). PubMed: 31509622